PXD003092

PXD003092 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics analysis of anti-cancer effects of ginsenoside Rg3 in human breast cancer cells
Description	The breast cancer incidence has been increasing in China, with the earlier age of onset compared with Western countries. Traditional Chinese medicine has been provided as one of the major source of anti-cancer drugs. Ginseng is one of the most common traditional medicines in China. Ginsenosides, the saponins in the plant Panax (ginseng) are the major active components responsible for their chemopreventive effects from cancer. However, the mechanisms by which ginsenosides exert their anti-cancer effect remain elusive. By combining TMT-based quantitation with TiO2-based phosphopeptide enrichment, we performed a quantitative analysis of the changes of the phosphoproteomes in ginsenoside Rg3-treated breast cancer MDA-MB-231 cells. We were able to quantitate 5,140 phosphorylation sites on 2,041 phosphoproteins. Our data show that the phosphorylation status of 13 sites was changed in MDA-MB-231 cells upon Rg3 treatment. The perturbed phosphoproteins are CPSF7, EEF2, HIRIP3, MAGED2, MPRIP, MYCBP2, PAWR, PPP1R12A, RANBP2, SEPT9, TMPO, and UFL1. These proteins are involved in various biological processes, including protein synthesis, cell division, and inhibition of NF-κB signaling. Our study revealed that Rg3 exerts its anti-cancer effects through a combination of different signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2018-01-08
AnnouncementXML	Submission_2018-05-23_01:38:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Fang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-21 01:58:21	ID requested
1	2018-01-08 01:09:11	announced
⏵ 2	2018-05-23 01:38:50	announced	Updated publication reference for PubMed record(s): 29435015.

Zou M, Wang J, Gao J, Han H, Fang Y, Phosphoproteomic analysis of the antitumor effects of ginsenoside Rg3 in human breast cancer cells. Oncol Lett, 15(3):2889-2898(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Ginsenoside Rg3, Triple negative breast cancer, phosphorylation, MDA-MB-231, 6-plex TMT, LC-MS/MS, CPSF7, EEF2, HIRIP3, MAGED2, MPRIP, MYCBP2, PAWR, PPP1R12A, RANBP2, SEPT9, TMPO, UFL1

Hui Han
contact affiliation	Department of Infection Control, Qilu Hospital, Shandong University, Jinan, Shandong, People’s Republic of China
contact email	hanhui.sdu@gmail.com
lab head
Yi Fang
contact affiliation	Chinese Academy of Medical Sciences and Peking Union Medical College
contact email	fangyi.cicams@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD003092

PRIDE project URI

• PRIDE
  1. PXD003092
     1. Label: PRIDE project
     2. Name: Phosphoproteomics analysis of anti-cancer effects of ginsenoside Rg3 in human breast cancer cells