PXD003089

PXD003089 is an original dataset announced via ProteomeXchange.

Title	Mouse SJ, LC-MS/MS
Description	Learning and memory processes are accompanied by rearrangements of synaptic protein networks. While various studies have demonstrated the regulation of individual synaptic proteins during these processes, much less is known about the complex regulation of entire synaptic proteomes. Recently, we reported that auditory discrimination learning in mice is associated with a relative down-regulation of proteins involved in the structural organization of synapses in various brain regions. Aiming at the identification of biological processes and signaling pathways involved in auditory memory formation, here a label-free quantification approach was utilized to identify regulated synaptic junctional proteins and phosphoproteins in the auditory cortex, frontal cortex, hippocampus and striatum of mice 24 h after the learning experiment. Twenty proteins, including postsynaptic scaffolds, actin modeling proteins and RNA-binding proteins, were regulated in at least three brain regions pointing to common, cross-regional mechanisms. Most of the detected synaptic proteome changes were, however, restricted to individual brain regions. For example, several members of the Septin family of cytoskeletal proteins were upregulated only in the hippocampus, while Septin-9 was down-regulated in the hippocampus, the frontal cortex and the striatum. Meta analyses utilizing several databases were employed to identify underlying cellular functions and biological pathways.
HostingRepository	PRIDE
AnnounceDate	2016-04-14
AnnouncementXML	Submission_2016-04-14_02:33:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003089
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thilo Kaehne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-20 23:59:09	ID requested
⏵ 1	2016-04-14 02:33:19	announced

K, ä, hne T, Richter S, Kolodziej A, Smalla KH, Pielot R, Engler A, Ohl FW, Dieterich DC, Seidenbecher C, Tischmeyer W, Naumann M, Gundelfinger ED, Proteome rearrangements after auditory learning: high-resolution profiling of synapse-enriched protein fractions from mouse brain. J Neurochem, 138(1):124-38(2016) [pubmed]

curator keyword: Biological

submitter keyword: Auditory learning , label-free quantification, phosphoproteomics, learning and memory , quantitative mass spectrometry, chemical synapse

Thilo Kaehne
contact affiliation	University Magdeburg, Inst. Exp. Internal Medicine Leipziger Str.44 39120 Magdeburg Germany
contact email	kaehne@med.ovgu.de
lab head
Thilo Kaehne
contact affiliation	University Magdeburg, Germany
contact email	kaehne@med.ovgu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003089

PRIDE project URI

• PRIDE
  1. PXD003089
     1. Label: PRIDE project
     2. Name: Mouse SJ, LC-MS/MS