PXD003086

PXD003086 is an original dataset announced via ProteomeXchange.

Title	Effect of cigarette smoke on microRNA expression in H292 lung cancer cells
Description	Cigarette smoke has been associated with the development of various lung diseases including cancer. Dysregulation of miRNAs is known to affect protein expression which leads to diverse functional consequences. Investigating miRNA and protein expression in response to cigarette smoke exposure can lead to the identification of potential therapeutic and chemopreventive targets. We employed a SILAC-based quantitative proteomic analysis to identify proteins differentially expressed in response to cigarette smoke in H292 lung cancer cells. LC-MS/MS analysis led to the identification of 3,959 proteins, of which, 303 proteins were overexpressed and 112 proteins were found to be downregulated in cigarette smoke-treated H292 cells.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_04:02:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-20 07:14:15	ID requested
⏵ 1	2018-10-25 04:02:19	announced

Advani J, Subbannayya Y, Patel K, Khan AA, Patil AH, Jain AP, Solanki HS, Radhakrishnan A, Pinto SM, Sahasrabuddhe NA, Thomas JK, Mathur PP, Nair BG, Chang X, Prasad TSK, Sidransky D, Gowda H, Chatterjee A, Long-Term Cigarette Smoke Exposure and Changes in MiRNA Expression and Proteome in Non-Small-Cell Lung Cancer. OMICS, 21(7):390-403(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Lung cancer, mass spectrometry, miRNA

Akhilesh Pandey
contact affiliation	McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland 21205 USA
contact email	pandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003086

PRIDE project URI

• PRIDE
  1. PXD003086
     1. Label: PRIDE project
     2. Name: Effect of cigarette smoke on microRNA expression in H292 lung cancer cells