PXD003084

PXD003084 is an original dataset announced via ProteomeXchange.

Title	Absolute quantification of budding yeast proteome with PCS method
Description	Absolute quantification of proteome is one of the most important tasks in proteomic research. The aim in this analysis is providing proof-of-principle of our originally developed strategy for absolute quantification of multiple proteins present at various concentrations in a cell, using stable isotope-labeled peptide concatenated standard (PCS). This study also provides insights into structures of regulation of metabolic pathway and into biological mechanisms for regulation of expressed protein abundance.
HostingRepository	PRIDE
AnnounceDate	2016-10-06
AnnouncementXML	Submission_2016-10-06_02:18:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keiji Kito
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-20 06:31:06	ID requested
⏵ 1	2016-10-06 02:18:08	announced

Kito K, Okada M, Ishibashi Y, Okada S, Ito T, A strategy for absolute proteome quantification with mass spectrometry by hierarchical use of peptide-concatenated standards. Proteomics, 16(10):1457-73(2016) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Budding yeast, Absolute quantification, Peptide-concatenated standard (PCS)

Keiji Kito
contact affiliation	School of Agriculture, Meiji University, Japan
contact email	Kito@meiji.ac.jp
lab head
Keiji Kito
contact affiliation	Meiji University
contact email	kito@meiji.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003084

PRIDE project URI

• PRIDE
  1. PXD003084
     1. Label: PRIDE project
     2. Name: Absolute quantification of budding yeast proteome with PCS method