PXD003080

PXD003080 is an original dataset announced via ProteomeXchange.

Title	Changes in liver proteins of rats fed both low and high-fat and sucrose diets induced by fish omega-3 PUFAs and their combination with grape polyphenols according to quantitative proteomics, part 2
Description	This study considers the physiological modulation of liver proteins due to the supplementation with fish oils under two different dietary backgrounds: low- or high- fat and sucrose diets, and the effect of their combination with an antioxidant agent (grape polyphenols) which provides reducing power. For this scope, a quantitative proteomics approach based on the Isobaric Tag for relative and Absolute Quantitation methodology (iTRAQ)-coupled to nano-LC-MS/MS and complemented with 2D-DIGE analysis were used for determining the regulation of liver proteins exerted by the supplementation with fish oils, polyphenols or their combination of Wistar Kyoto rats in the two chosen dietary backgrounds. This experimental design was useful to investigate if the behavior of fish oils changes when the dietary background is modified and the possible synergy between fish oils and polyphenols. Results show that the capacity of fish oils, polyphenols or their combination for down or up-regulating liver proteins depends on the dietary context. In the background of low-fat low-sucrose healthy diets, 10 different proteins were altered by the sum of three supplements, in opposite to the 45 altered proteins found in the high-fat high-sucrose unhealthy diets. In both situations, fish oils seemed to be the main force for regulating liver proteins, although the addition of polyphenols was able to modulate some fish oils effects. Moreover, we provide evidence of the effect of fish oils and their combination with grape polyphenols for improving biochemical parameters and for reducing enzymes of hepatic lipogenesis and glycolysis, for enhancing fatty acid beta oxidation and insulin signaling and for the amelioration of endoplasmic reticule stress and protein oxidation when are included in an unhealthy diet.
HostingRepository	PRIDE
AnnounceDate	2017-08-02
AnnouncementXML	Submission_2017-08-02_09:27:55.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003080
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-19 02:47:27	ID requested
1	2017-01-17 12:16:30	announced
⏵ 2	2017-08-02 09:27:56	announced	Updated project metadata.

M, é, ndez L, Ciordia S, Fern, á, ndez MS, Ju, á, rez S, Ramos A, Pazos M, Gallardo JM, Torres JL, Nogu, é, s MR, Medina I, Changes in liver proteins of rats fed standard and high-fat and sucrose diets induced by fish omega-3 PUFAs and their combination with grape polyphenols according to quantitative proteomics. J Nutr Biochem, 41():84-97(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Liver, high-fat, sucrose, diet, omega-3, proteomics, 2D-DIGE

Isabel Medina
contact affiliation	Instituto de Investigaciones Marinas (IIM-CSIC)
contact email	medina@iim.csic.es
lab head
Sergio Ciordia
contact affiliation	National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003080
     1. Label: PRIDE project
     2. Name: Changes in liver proteins of rats fed both low and high-fat and sucrose diets induced by fish omega-3 PUFAs and their combination with grape polyphenols according to quantitative proteomics, part 2