PXD003078

PXD003078 is an original dataset announced via ProteomeXchange.

Title	Recombinant shewasin D and shewasin A specificity preferences profiled by proteomic identification of protease cleavage sites (PICS)
Description	We determined the specificity preferences of both recombinant shewasin D and shewasin A (pepsin-like aspartic proteases from S. denitrificans and S. amazonensis, respectively) using proteome-derived peptide libraries and observed remarkable similarities between both shewasins and eukaryotic pepsins, in particular with BACE-1 thereby confirming their phylogenetic proximity. We further observed subtle differences in sequence preferences at several positions, suggesting variations in their subsite binding pockets.
HostingRepository	PRIDE
AnnounceDate	2016-04-05
AnnouncementXML	Submission_2016-04-05_02:27:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Cruz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	thioacylation of primary amines - site N-term; biotinylated residue; thioacylation of primary amines - site K; dimethylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-19 02:08:23	ID requested
⏵ 1	2016-04-05 02:27:20	announced

Leal AR, Cruz R, Bur D, Huesgen PF, Faro R, Manadas B, Wlodawer A, Faro C, Sim, õ, es I, Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans. Sci Rep, 6():23869(2016) [pubmed]

curator keyword: Biological

submitter keyword: PICS

trypsin

GluC

shewasin D

shewasin A

Shewanella denitrificans

Shewanella amazonensis

pepsin-like

specificity profile;

Isaura Simões
contact affiliation	CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal; Biocant, Biotechnology Innovation Center, 3060-197 Cantanhede, Portugal;
contact email	isimoes@biocant.pt
lab head
Rui Cruz
contact affiliation	Center for Neuroscience and Cell Biology
contact email	ruicruzcc@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003078

PRIDE project URI

• PRIDE
  1. PXD003078
     1. Label: PRIDE project
     2. Name: Recombinant shewasin D and shewasin A specificity preferences profiled by proteomic identification of protease cleavage sites (PICS)