PXD003073

PXD003073 is an original dataset announced via ProteomeXchange.

Title	A systems study reveals concurrent activation of AMPK and mTOR by amino acids
Description	Amino acids (aa) are not only building blocks for proteins, but also signalling molecules, with the mammalian target of rapamycin complex 1 (mTORC1) acting as a key mediator. However, little is known about whether aa, independently of mTORC1, activate other kinases of the mTOR signalling network. To delineate aa-stimulated mTOR network dynamics, we here combine a computational–experimental approach with text mining-enhanced quantitative proteomics. We report that AMP-activated protein kinase (AMPK), phosphatidylinositide 3-kinase (PI3K) and mTOR complex 2 (mTORC2) are acutely activated by aa-readdition in an mTORC1-independent manner. AMPK activation by aa is mediated by Ca2+/calmodulin-dependent protein kinase kinase β (CaMKKβ). In response, AMPK impinges on the autophagy regulators Unc-51-like kinase-1 (ULK1) and c-Jun. AMPK is widely recognized as an mTORC1 antagonist that is activated by starvation. We find that aa acutely activate AMPK concurrently with mTOR. We show that AMPK under aa sufficiency acts to sustain autophagy. This may be required to maintain protein homoeostasis and deliver metabolite intermediates for biosynthetic processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:59.836.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Horvatovich Péter
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-19 01:29:49	ID requested
1	2016-12-12 05:22:30	announced
2	2017-10-24 02:16:59	announced	Updated project metadata.
⏵ 3	2024-10-22 04:34:04	announced	2024-10-22: Updated project metadata.

10.1038/ncomms13254;

Dalle Pezze P, Ruf S, Sonntag AG, Langelaar-Makkinje M, Hall P, Heberle AM, Razquin Navas P, van Eunen K, T, ö, lle RC, Schwarz JJ, Wiese H, Warscheid B, Deitersen J, Stork B, F, ä, ß, ler E, Sch, ä, uble S, Hahn U, Horvatovich P, Shanley DP, Thedieck K, A systems study reveals concurrent activation of AMPK and mTOR by amino acids. Nat Commun, 7():13254(2016) [pubmed]

curator keyword: Biological

submitter keyword: system modelling, AMPK, SILAC,mTOR, phosphoproteomics

Kathrin Thedieck
contact affiliation	Lab for Metabolic Signaling, Department of Pediatrics, University of Groningen, University Medical Center Groningen (UMCG), 9713 AV Groningen, The Netherlands and School of Medicine and Health Sciences, Carl von Ossietzky University Oldenburg, 26111 Oldenburg, Germany
contact email	k.thedieck@umcg.nl
lab head
Horvatovich Péter
contact affiliation	Faculty of Science and Engineering, Groningen Research Institute of Pharmacy, Analytical Biochemistry, University of Groningen, 9713 AV Groningen, The Netherlands
contact email	p.l.horvatovich@rug.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003073

PRIDE project URI

• PRIDE
  1. PXD003073
     1. Label: PRIDE project
     2. Name: A systems study reveals concurrent activation of AMPK and mTOR by amino acids