<<< Full experiment listing

PXD003063

PXD003063 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffects of the antibacterial compund Allicin from garlic on Escherichia coli
DescriptionAllicin (diallyl thiosulfinate) from garlic is a highly potent natural antimicrobial substance. It inhibits growth of a variety of microorganisms, among them antibiotic resistant strains. Here, we show that upon exposure to allicin, growth of Escherichia coli is strongly inhibited. Growth inhibition coincides with a significant drop of total sulfhydryl concentrations and induction of the heat shock and oxidative stress response. In contrast to diamide, a potent inducer of disulfide stress, allicin does not induce cystine bond formation in proteins such as cytoplasmically expressed alkaline phosphatase PhoA. We identified and quantified the allicin-induced modification S-allylmercapto-cysteine for a set of cytoplasmic proteins by using a combination of label-free mass spectrometry and differential OxICAT labeling. Activity of isocitrate lyase AceA, an S-allylmercapto-modified candidate protein, is largely inhibited by allicin treatment in vivo. Our results indicate that the mode of action of allicin is a combination of disturbance of the redox balance and inactivation of crucial metabolic enzymes.
HostingRepositoryPRIDE
AnnounceDate2016-05-02
AnnouncementXMLSubmission_2016-05-02_05:31:02.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLars Leichert
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListApplied Biosystems cleavable ICAT(TM) light; Applied Biosystems cleavable ICAT(TM) heavy
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-10-16 03:12:13ID requested
12016-05-02 05:31:05announced
Publication List
M, ü, ller A, Eller J, Albrecht F, Prochnow P, Kuhlmann K, Bandow JE, Slusarenko AJ, Leichert LI, Allicin Induces Thiol Stress in Bacteria through S-Allylmercapto Modification of Protein Cysteines. J Biol Chem, 291(22):11477-90(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Escherichia coli, Allicin, Thiol, S-Allylmercapto
Contact List
Lars Ingo Ole Leichert
contact affiliationRuhr-Universität Bochum Institute of Biochemistry and Pathobiochemistry Microbial Biochemistry Universitätsstrasse 150 44780 Bochum, Germany
contact emaillars.leichert@ruhr-uni-bochum.de
lab head
Lars Leichert
contact affiliationRuhr-Universität Bochum
contact emaillars.leichert@ruhr-uni-bochum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD003063
PRIDE project URI
Repository Record List
[ + ]