Updated project metadata. The Sertoli cells (Sc) of 5 days (infant) and 12 days (pubertal) old rat were isolated and cultured in triplicates. Nuclear and cytoplasmic fractionation was done for both the cases. All the four protein fractions (nuclear and cytoplasm of infant and pubertal Sc) were anaysed using Lc-MS/MS. SWATH analysis was done for all the four samples in biological and technical replicates. The objective was to quantity the proteins of all the samples with respect to each other at whole proteome level.