PXD003053 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HYPE mediated AMPylation site profiling with YnATP in human lysates |
Description | We describe here the first example of global chemoproteomic screening for HYPE mediated AMPylation sites of human proteins. Catalytically active mutant HYPE E234G was applied in combination with an alkynylated ATP and an alkynyl-biotinylated side ID reagent for capture of chemoenzymatically tagged proteins. AMPylation sites were detected by shotgun proteomics performed on a Q-Exactive instrument. |
HostingRepository | PRIDE |
AnnounceDate | 2015-12-02 |
AnnouncementXML | Submission_2015-12-02_03:28:03.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003053 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Remigiusz Serwa |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-14 06:35:58 | ID requested | |
⏵ 1 | 2015-12-02 03:28:04 | announced | |
Publication List
Broncel M, Serwa RA, Bunney TD, Katan M, Tate EW, Global Profiling of Huntingtin-associated protein E (HYPE)-Mediated AMPylation through a Chemical Proteomic Approach. Mol Cell Proteomics, 15(2):715-25(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, chemoproteomics, protein AMPylation |
Contact List
Edward Tate |
contact affiliation | Department of Chemistry, Imperial College London |
contact email | e.tate@imperial.ac.uk |
lab head | |
Remigiusz Serwa |
contact affiliation | Imperial College London |
contact email | r.serwa@imperial.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003053
- Label: PRIDE project
- Name: HYPE mediated AMPylation site profiling with YnATP in human lysates