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PXD003053

PXD003053 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHYPE mediated AMPylation site profiling with YnATP in human lysates
DescriptionWe describe here the first example of global chemoproteomic screening for HYPE mediated AMPylation sites of human proteins. Catalytically active mutant HYPE E234G was applied in combination with an alkynylated ATP and an alkynyl-biotinylated side ID reagent for capture of chemoenzymatically tagged proteins. AMPylation sites were detected by shotgun proteomics performed on a Q-Exactive instrument.
HostingRepositoryPRIDE
AnnounceDate2015-12-02
AnnouncementXMLSubmission_2015-12-02_03:28:03.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003053
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRemigiusz Serwa
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-10-14 06:35:58ID requested
12015-12-02 03:28:04announced
Publication List
Broncel M, Serwa RA, Bunney TD, Katan M, Tate EW, Global Profiling of Huntingtin-associated protein E (HYPE)-Mediated AMPylation through a Chemical Proteomic Approach. Mol Cell Proteomics, 15(2):715-25(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Human, chemoproteomics, protein AMPylation
Contact List
Edward Tate
contact affiliationDepartment of Chemistry, Imperial College London
contact emaile.tate@imperial.ac.uk
lab head
Remigiusz Serwa
contact affiliationImperial College London
contact emailr.serwa@imperial.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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