PXD003049

PXD003049 is an original dataset announced via ProteomeXchange.

Title	Identification of calredoxin (C. reinhardtii) interaction partners by affinity chromatography and LC-MS/MS
Description	Calcium (Ca2+) and redox signaling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein from Chlamydomonas reinhardtii that has the competence to integrate Ca2+- and redox-related signaling. This protein, designated as calredoxin (CRX), combines four Ca2+-binding EF-hands and a thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold of the Ca2+-binding EF-hands, which is functionally linked via an inter-domain communication path with the enzymatically active TRX domain. CRX is chloroplast-localized and interacted with a chloroplast 2-Cys-peroxiredoxin (PRX1). Ca2+-binding to CRX is critical for its TRX activity and for efficient binding and reduction of PRX1. Thereby, CRX represents a new class of Ca2+-dependent "sensor-responder" proteins. Genetically engineered Chlamydomonas strains with strongly diminished amounts of CRX revealed altered photosynthetic electron transfer and were affected in oxidative stress response underpinning a function of CRX in stress acclimation.
HostingRepository	PRIDE
AnnounceDate	2016-10-12
AnnouncementXML	Submission_2016-10-12_03:46:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Scholz
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-13 07:23:14	ID requested
⏵ 1	2016-10-12 03:46:59	announced

Hochmal AK, Zinzius K, Charoenwattanasatien R, G, ä, belein P, Mutoh R, Tanaka H, Schulze S, Liu G, Scholz M, Nordhues A, Offenborn JN, Petroutsos D, Finazzi G, Fufezan C, Huang K, Kurisu G, Hippler M, Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast. Nat Commun, 7():11847(2016) [pubmed]

curator keyword: Biological

submitter keyword: Chlamydomonas, calredoxin

Michael Hippler
contact affiliation	WWU Muenster, Institute of Plant Biology and Biotechnology
contact email	mhippler@uni-muenster.de
lab head
Martin Scholz
contact affiliation	IBBP Muenster
contact email	mscholz@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003049
     1. Label: PRIDE project
     2. Name: Identification of calredoxin (C. reinhardtii) interaction partners by affinity chromatography and LC-MS/MS