PXD003040

PXD003040 is an original dataset announced via ProteomeXchange.

Title	The SILAC fly allows for accurate protein quantification in vivo
Description	Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level was the laboratory mouse. The fruit fly Drosophila melanogaster is one of the most widely used small animal models in biology. Here, we show that feeding flies with SILAC-labeled yeast leads to almost complete labeling in the first filial generation. We used these "SILAC flies" to investigate sexual dimorphism of protein abundance in D. melanogaster. Quantitative proteome comparison of adult male and female flies revealed distinct biological processes specific for each sex. Using a tudor mutant that is defective for germ cell generation allowed us to differentiate between sex-specific protein expression in the germ line and somatic tissue. We identified many proteins with known sex-specific expression bias. In addition, several new proteins with a potential role in sexual dimorphism were identified. Collectively, our data show that the SILAC fly can be used to accurately quantify protein abundance in vivo. The approach is simple, fast, and cost-effective, making SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.
HostingRepository	PRIDE
AnnounceDate	2015-10-12
AnnouncementXML	Submission_2015-10-12_06:37:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia-Xuan Chen
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-12 04:19:17	ID requested
⏵ 1	2015-10-12 06:37:51	announced

Sury MD, Chen JX, Selbach M, The SILAC fly allows for accurate protein quantification in vivo. Mol Cell Proteomics, 9(10):2173-83(2010) [pubmed]

submitter keyword: in vivo, SILAC, Drosophila

Matthias Selbach
contact affiliation	Max Delbrück Center for Molecular Medicine
contact email	matthias.selbach@mdc-berlin.de
lab head
Jia-Xuan Chen
contact affiliation	Max Delbrück Center for Molecular Medicine
contact email	jiaxuan.chen@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003040
     1. Label: PRIDE project
     2. Name: The SILAC fly allows for accurate protein quantification in vivo