PXD003031

PXD003031 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome
Description	Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome We determined 1) the phosphorylation sites within the Asc1 protein purified from Saccharomyces cerevisiae via its Strep-tag and 2) the Asc1p-dependent phospho-proteome. 1) Phospho-sites within tryptic peptides of Asc1p were identified using the Proteome Discoverer 1.4 software with the SequestHT and Mascot search engines and phosphorylation site localization was evaluated with the phosphoRS tool. 2) Quantitative triple SILAC-based phospho-proteome comparison of an ASC1 wild-type strain with asc1 mutant strains. MS data were analyzed with MaxQuant and Perseus.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:41:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-12 00:57:16	ID requested
1	2016-12-13 08:14:26	announced
⏵ 2	2017-10-24 02:41:38	announced	Updated project metadata.

Schmitt K, Smolinski N, Neumann P, Schmaul S, Hofer-Pretz V, Braus GH, Valerius O, Asc1p/RACK1 Connects Ribosomes to Eukaryotic Phosphosignaling. Mol Cell Biol, 37(3):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae, Asc1p, RACK1, LC-MSMS, SILAC, signal transduction, translation, phosphorylation

Dr. Oliver Valerius
contact affiliation	Georg-August-University Göttingen Department of Molecular Microbiology and Genetics Institute of Microbiology and Genetics
contact email	ovaleri@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003031
     1. Label: PRIDE project
     2. Name: Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome