PXD003025

PXD003025 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of the infectious and replicative forms of Chlamydia trachomatis L2
Description	The obligate intracellular developmental cycle of Chlamydia trachomatis presents significant challenges in defining its proteome. In this study we have applied quantitative proteomics to both the intracellular reticulate body (RB) and the extracellular elementary body (EB) from C. trachomatis. We used C. trachomatis L2 which is a model chlamydial isolate for such a study since it has a high infectivity: particle ratio and there is an excellent quality genome sequence. EBs and RBs (>99% pure) were quantified by chromosomal and plasmid copy number using PCR to determine the concentrations of chlamydial proteins per bacterial cell. RBs harvested at 15h post infection (PI) were purified by three successive rounds of gradient centrifugation. This is the earliest possible time to obtain purified RBs, free from host cell components in quantity, within the constraints of the technology, EBs were purified at 48h PI. We then used two-dimensional reverse phase UPLC to fractionate RB or EB peptides before mass spectroscopic analysis, providing absolute amount estimates of chlamydial proteins.
HostingRepository	PRIDE
AnnounceDate	2016-06-28
AnnouncementXML	Submission_2016-06-28_07:38:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Skipp
SpeciesList	scientific name: Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B); NCBI TaxID: 471472;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-09 02:32:37	ID requested
⏵ 1	2016-06-28 07:38:19	announced

Skipp PJ, Hughes C, McKenna T, Edwards R, Langridge J, Thomson NR, Clarke IN, Quantitative Proteomics of the Infectious and Replicative Forms of Chlamydia trachomatis. PLoS One, 11(2):e0149011(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Chlamydia trachomatis, Reticulate body, Elementary body, L2

Paul James Skipp
contact affiliation	Centre for Proteomic Research, University of Southampton
contact email	pjss@soton.ac.uk
lab head
Paul Skipp
contact affiliation	Centre for Proteomic Research, University of Southampton
contact email	pjss@soton.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003025
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of the infectious and replicative forms of Chlamydia trachomatis L2