PXD003022

PXD003022 is an original dataset announced via ProteomeXchange.

Title	Tetranychus urticae secretome
Description	The two-spotted spider mite Tetranychus urticae is an extreme polyphaguous crop pest. Next to an increased detoxification potential of plant secondary metabolites, it has recently been shown that spider mites manipulate plant defences. Salivary constituents are proposed to play an important role during the interaction with its many hosts. The proteomic composition of saliva delivered into artificial diet by spider mites adapted to various hosts - bean, soy, maize, tomato -was determined using Orbitrap mass spectrometry. Over 200 different proteins were identified, many of unknown function and in numerous cases belonging to multi-membered gene families. A selection of these putative salivary proteins was validated using whole-mount in situ hybridizations and expression was shown to be localized in the anterior and dorsal podocephalic glands of the spider mite. Host-plant dependent expression was evident from the proteomics dataset and was further studied in detail by micro-array based genome wide gene expression profiling of mites maintained on the host plants under study. Previously obtained gene-expression datasets were further used to get more insight in the expression profile over different life stages and physiological states. To conclude, for the first time the T. urticae salivary proteome repertoire was characterized using a custom feeding hemisphere-based enrichment technique. This knowledge will assist in unraveling the molecular interactions between phytophagous mites and their host plants. This may ultimately facilitate the development of mite-resistant crops.
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-02-06_01:13:55.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003022
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerben Menschaert
SpeciesList	scientific name: Tetranychus urticae (Two-spotted spider mite); NCBI TaxID: 32264;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-08 02:53:37	ID requested
1	2017-01-17 03:04:10	announced
⏵ 2	2017-02-06 01:13:57	announced	Updated publication reference for PubMed record(s): 27703040.

Jonckheere W, Dermauw W, Zhurov V, Wybouw N, Van den Bulcke J, Villarroel CA, Greenhalgh R, Grbi, ć M, Schuurink RC, Tirry L, Baggerman G, Clark RM, Kant MR, Vanholme B, Menschaert G, Van Leeuwen T, The Salivary Protein Repertoire of the Polyphagous Spider Mite Tetranychus urticae: A Quest for Effectors. Mol Cell Proteomics, 15(12):3594-3613(2016) [pubmed]

curator keyword: Biological

submitter keyword: Tetranichus urticae, secretome,

Gerben Menschaert
contact affiliation	BioBix, Lab for Bioinformatics and Computational Genomics, Ghent University, Belgium
contact email	Gerben.Menschaert@gmail.com
lab head
Gerben Menschaert
contact affiliation	Faculty of Bioscience Engineering
contact email	gerben.menschaert@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD003022

PRIDE project URI

• PRIDE
  1. PXD003022
     1. Label: PRIDE project
     2. Name: Tetranychus urticae secretome