PXD003005

PXD003005 is an original dataset announced via ProteomeXchange.

Title	Mouse wild-type and Anxa5-null utricles and hair bundles
Description	The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse vestibular hair bundles, present at ~15,000 copies per stereocilium, which is ~2% of the total protein there. Although seven other annexin genes are expressed in mouse utricles, mass spectrometry showed that none were upregulated in stereocilia of Anxa5-/- mice. Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism in hair cells after noise damage. Nevertheless, Anxa5-/- mice not only have normal hearing or balance, but are identical to wild-type mice in their temporary or permanent hearing changes following noise exposure. We suggest that the significant amounts of ANXA2 and ANXA6 that are present in the hair-cell cytoplasm can substitute in any function carried out by ANXA5, and that ANXA5 may be playing a hitchhiker role in the bundle. These results demonstrate that abundance of a protein in a specific compartment or cellular structure does not necessarily imply functional significance.
HostingRepository	PRIDE
AnnounceDate	2016-06-09
AnnouncementXML	Submission_2016-06-09_07:56:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barr-Gillespie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-05 01:48:24	ID requested
⏵ 1	2016-06-09 07:56:52	announced

Krey JF, Drummond M, Foster S, Porsov E, Vijayakumar S, Choi D, Friderici K, Jones SM, Nuttall AL, Barr-Gillespie PG, Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and Function. Sci Rep, 6():27221(2016) [pubmed]

submitter keyword: hair cells, stereocilia, annexins, vestibular

Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239
contact email	gillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center
contact email	gillespp@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003005

PRIDE project URI

• PRIDE
  1. PXD003005
     1. Label: PRIDE project
     2. Name: Mouse wild-type and Anxa5-null utricles and hair bundles