PXD003001

PXD003001 is an original dataset announced via ProteomeXchange.

Title	Triple SILAC exosomal quantitative proteomic analysis
Description	Exosomes are 30-100 nm sized membrane vesicles released by cells into extracellular space and mediate the intercellular communication via transfer of proteins and RNAs. To better understand the function of these microvesicles in lung carcinogenesis, we employed a Triple SILAC quantitative proteomic strategy to examine the differential protein abundance between exosomes derived from an immortalized normal bronchial epithelial cell line and two non-small cell lung cancer (NSCLC) cell lines harboring distinct activating mutations in the cell signaling molecules Kirsten rat sarcoma viral oncogene homolog (KRAS) or epidermal growth factor receptor (EGFR). We were able to quantify 727 exosomal proteins derived from the three cell lines. Proteins associated with signal transduction are enriched in NSCLC exosomes which are functionally active in regulating cell proliferation. The study is the first to investigate protein abundance differences in exosomes derived from NSCLC cells and their normal counterparts, and reveals the role of exosomes in NSCLC cancer progression, which may have clinical implications in biomarker development for patients with NSCLC.
HostingRepository	PRIDE
AnnounceDate	2016-01-12
AnnouncementXML	Submission_2016-01-12_06:30:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Clark
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-02 02:36:54	ID requested
⏵ 1	2016-01-12 06:30:41	announced

Clark DJ, Fondrie WE, Yang A, Mao L, Triple SILAC quantitative proteomic analysis reveals differential abundance of cell signaling proteins between normal and lung cancer-derived exosomes. J Proteomics, 133():161-169(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Exosomes, non-small cell lung cancer, SILAC, quantitative proteomics

Li Mao
contact affiliation	Department of Oncology and Diagnostics Sciences University of Maryland School of Dentistry Baltimore, MD
contact email	lmao@umaryland.edu
lab head
David Clark
contact affiliation	UMB School of Dentistry
contact email	david.clark@umaryland.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003001
     1. Label: PRIDE project
     2. Name: Triple SILAC exosomal quantitative proteomic analysis