PXD003000 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemoproteomic profiling of host and pathogen enzymes active in cholera |
Description | Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human cholera stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and rabbit enzymes in vivo, while genetic disruption of all four proteases increased the abundance and binding of an intestinal lectin—intelectin—to V. cholerae in infected rabbits. Intelectin also bound to other enteric bacterial pathogens, suggesting it may constitute a previously unrecognized mechanism of bacterial surveillance in the intestine that is inhibited by pathogen-secreted proteases. Our work demonstrates the power of activity-based proteomics to reveal host-pathogen enzymatic dialogue in an animal model of infection. |
HostingRepository | PRIDE |
AnnounceDate | 2016-02-22 |
AnnouncementXML | Submission_2016-02-25_01:20:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eranthie Weerapana |
SpeciesList | scientific name: Vibrio cholerae O1 str. VC91; NCBI TaxID: 1175271; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-02 02:28:26 | ID requested | |
1 | 2016-02-22 07:33:16 | announced | |
⏵ 2 | 2016-02-25 01:20:21 | announced | Updated publication reference for PubMed record(s): 26900865. |
Publication List
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK, Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nat Chem Biol, 12(4):268-274(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Vibrio cholerae |
rabbit |
Contact List
Eranthie Weerapana |
contact affiliation | Boston College |
contact email | eranthie@bc.edu |
lab head | |
Eranthie Weerapana |
contact affiliation | Boston College |
contact email | eranthie@bc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003000 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003000
- Label: PRIDE project
- Name: Chemoproteomic profiling of host and pathogen enzymes active in cholera