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PXD003000

PXD003000 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemoproteomic profiling of host and pathogen enzymes active in cholera
DescriptionActivity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human cholera stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and rabbit enzymes in vivo, while genetic disruption of all four proteases increased the abundance and binding of an intestinal lectin—intelectin—to V. cholerae in infected rabbits. Intelectin also bound to other enteric bacterial pathogens, suggesting it may constitute a previously unrecognized mechanism of bacterial surveillance in the intestine that is inhibited by pathogen-secreted proteases. Our work demonstrates the power of activity-based proteomics to reveal host-pathogen enzymatic dialogue in an animal model of infection.
HostingRepositoryPRIDE
AnnounceDate2016-02-22
AnnouncementXMLSubmission_2016-02-25_01:20:19.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEranthie Weerapana
SpeciesList scientific name: Vibrio cholerae O1 str. VC91; NCBI TaxID: 1175271;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-10-02 02:28:26ID requested
12016-02-22 07:33:16announced
22016-02-25 01:20:21announcedUpdated publication reference for PubMed record(s): 26900865.
Publication List
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK, Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nat Chem Biol, 12(4):268-274(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Vibrio cholerae
rabbit
Contact List
Eranthie Weerapana
contact affiliationBoston College
contact emaileranthie@bc.edu
lab head
Eranthie Weerapana
contact affiliationBoston College
contact emaileranthie@bc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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