PXD002999 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain |
Description | Nuclear proteins of developing wheat grains collected during the cellularization, effective grain-filling and maturation phases of development were analysed.. Nuclear proteins were extracted and separated by two-dimensional gel electrophoresis. Image analysis revealed 371 and 299 reproducible spots in gels with first dimension separation along pH 4-7 and pH 6-11 isoelectric gradients, respectively. The relative abundance of 464 (67%) protein spots changed during grain development. Abundance profiles of these proteins clustered in six groups associated with the major phases and phase transitions of grain development. Using nano liquid chromatography-tandem mass spectrometry to analyse 387 variant and non-variant protein spots, 114 different proteins were identified that were classified into 16 functional classes. We noted that some proteins involved in the regulation of transcription, like HMG1/2-like protein and histone deacetylase HDAC2, were most abundant before the phase transition from cellularization to grain-filling, suggesting that major transcriptional changes occur during this key developmental phase. The maturation period was characterized by high relative abundance of proteins involved in ribosome biogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2015-11-20 |
AnnouncementXML | Submission_2015-11-20_08:55:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Titouan Bonnot |
SpeciesList | scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; |
ModificationList | monohydroxylated residue; carbamoylated residue |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-02 00:31:32 | ID requested | |
⏵ 1 | 2015-11-20 08:55:17 | announced | |
Publication List
Bonnot T, Bancel E, Chambon C, Boudet J, Branlard G, Martre P, Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain. Front Plant Sci, 6():905(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Wheat, developing grain, nuclear proteins, LC-MS/MS |
Contact List
Emmanuelle Bancel |
contact affiliation | UMR 1095 GDEC, INRA Clermont Ferrand, Blaise Pascal University Aubière, FRANCE |
contact email | emmanuelle.bancel@clermont.inra.fr |
lab head | |
Titouan Bonnot |
contact affiliation | UMR GDEC |
contact email | titouan.bonnot@clermont.inra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD002999 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002999
- Label: PRIDE project
- Name: Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain