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PXD002999

PXD002999 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChanges in the nuclear proteome of developing wheat (Triticum aestivum L.) grain
DescriptionNuclear proteins of developing wheat grains collected during the cellularization, effective grain-filling and maturation phases of development were analysed.. Nuclear proteins were extracted and separated by two-dimensional gel electrophoresis. Image analysis revealed 371 and 299 reproducible spots in gels with first dimension separation along pH 4-7 and pH 6-11 isoelectric gradients, respectively. The relative abundance of 464 (67%) protein spots changed during grain development. Abundance profiles of these proteins clustered in six groups associated with the major phases and phase transitions of grain development. Using nano liquid chromatography-tandem mass spectrometry to analyse 387 variant and non-variant protein spots, 114 different proteins were identified that were classified into 16 functional classes. We noted that some proteins involved in the regulation of transcription, like HMG1/2-like protein and histone deacetylase HDAC2, were most abundant before the phase transition from cellularization to grain-filling, suggesting that major transcriptional changes occur during this key developmental phase. The maturation period was characterized by high relative abundance of proteins involved in ribosome biogenesis.
HostingRepositoryPRIDE
AnnounceDate2015-11-20
AnnouncementXMLSubmission_2015-11-20_08:55:16.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTitouan Bonnot
SpeciesList scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationListmonohydroxylated residue; carbamoylated residue
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-10-02 00:31:32ID requested
12015-11-20 08:55:17announced
Publication List
Bonnot T, Bancel E, Chambon C, Boudet J, Branlard G, Martre P, Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain. Front Plant Sci, 6():905(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Wheat, developing grain, nuclear proteins, LC-MS/MS
Contact List
Emmanuelle Bancel
contact affiliationUMR 1095 GDEC, INRA Clermont Ferrand, Blaise Pascal University Aubière, FRANCE
contact emailemmanuelle.bancel@clermont.inra.fr
lab head
Titouan Bonnot
contact affiliationUMR GDEC
contact emailtitouan.bonnot@clermont.inra.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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