PXD002998

PXD002998 is an original dataset announced via ProteomeXchange.

Title	DIA LC-MSMS of MCF-7 Breast Cancer Cells
Description	The breast cancer cell line MCF-7 was treated with doxorubicin. Protein expression differences between treated and non-treated cells were semiquantitatively determined using the precursor acquisition independent from ion count (PAcIFIC) mass spectrometric method. The acquired proteomic data sets were searched for regulated Reactome pathways and Gene Ontology annotation terms using a new algorithm (SetRank). Using this approach, we described significantly changed pathways (p-value of 0.05 or less) already known to be influenced by chemotherapeutic drugs, such as chromatin organization, DNA binding, embryo development, condensed chromosome, sequence-specific DNA binding, response to oxidative stress and response to toxin. Additionally, we found pathways such as central nervous system neuron differentiation, neuron projection membrane and SNAP receptor activity explaining side-effects of doxorubicin chemotherapy, characterized as ‘chemo brain’, on a molecular level.
HostingRepository	PRIDE
AnnounceDate	2015-11-09
AnnouncementXML	Submission_2015-11-09_00:01:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-02 00:14:39	ID requested
⏵ 1	2015-11-09 00:01:35	announced

Petrovic M, Simillion C, Kruzliak P, Sabo J, Heller M, Doxorubicin Affects Expression of Proteins of Neuronal Pathways in MCF-7 Breast Cancer Cells. Cancer Genomics Proteomics, 12(6):347-58(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: MCF-7, PAcIFIC, Data-independent acquisition, LC-MSMS

Manfred Heller
contact affiliation	Department of Clinical Research, University of Bern
contact email	manfred.heller@dkf.unibe.ch
lab head
Manfred Heller
contact affiliation	University of Bern
contact email	manfred.heller@dkf.unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002998
     1. Label: PRIDE project
     2. Name: DIA LC-MSMS of MCF-7 Breast Cancer Cells