PXD002997

PXD002997 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of Bordetella pertussis isolated from human macrophages
Description	Previous studies indicated that B. pertussis, historically considered as extracellular pathogen, can invade and replicate within human macrophages. In order to investigate factors involved in the intracellular survival, we analyzed the proteome of intracellular B. pertussis at 3h and 48 h post macrophage infection. From totally 762 identified proteins, we observed differences in gene expression profiles of intracellular bacteria as compared to extracellular bacteria. More than 300 proteins showed significant differences in the level of expression (p< 0.05) between internalized and non-internalized samples. Different intensities were found for proteins associated with virulence, stress response, cell division, transport, and many genes with unknown functions. Proteins involved in stress response increased in level early after infection, including ClpX, GroEL and HsIU. Two proteins involved in iron uptake were found increased as early as 3 h post infection and remained at high level 48 h post-infection. These proteins are BfrD and BfrE, recently identified as TonB-dependent outer membrane catecholamine receptors involved in iron uptake from transferrin in B. bronchiseptica. Interestingly, we found at least three proteins of the Type III Secretion System (TTSS) increased already at 3 h after infection, the proteins BteA, BscC, and BopN suggesting that TTSS becomes operative when bacteria infect human macrophages. Selected virulence factors and metabolic enzymes were validated using the multiple reaction monitoring. These results represent the first characterization of intracellular B. pertussis proteome and demonstrate that this pathogen undergoes an adaptive response that enables the development of an intracellular infection.
HostingRepository	PRIDE
AnnounceDate	2018-09-10
AnnouncementXML	Submission_2018-09-10_00:02:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristin Surmann
SpeciesList	scientific name: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); NCBI TaxID: 257313;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-01 23:57:20	ID requested
1	2016-02-29 04:22:33	announced
⏵ 2	2018-09-10 00:02:18	announced	Updated publication reference for PubMed record(s): 26873878, 30161230.

Cafiero JH, Lamberti YA, Surmann K, Vecerek B, Rodriguez ME, A Bordetella pertussis MgtC homolog plays a role in the intracellular survival. PLoS One, 13(8):e0203204(2018) [pubmed]

Lamberti Y, Cafiero JH, Surmann K, Valdez H, Holubova J, Ve, č, erek B, Sebo P, Schmidt F, V, ö, lker U, Rodriguez ME, Proteome analysis of Bordetella pertussis isolated from human macrophages. J Proteomics, 136():55-67(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: B. pertussis, macrophages, internalization, proteomics

Frank Schmidt
contact affiliation	University Medicine Greifswald, Germany
contact email	frank.schmidt@uni-greifswald.de
lab head
Kristin Surmann
contact affiliation	University Medicine Greifswald
contact email	surmannk@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002997

PRIDE project URI

• PRIDE
  1. PXD002997
     1. Label: PRIDE project
     2. Name: Proteome analysis of Bordetella pertussis isolated from human macrophages