PXD002996

PXD002996 is an original dataset announced via ProteomeXchange.

Title	Identification of syntrophic acetate-oxidizing bacteria in anaerobic digesters
Description	Inhibition of the anaerobic digestion process through accumulation of volatile fatty acids (VFA) is a recurring problem which is the result of unbalanced growth between acidogenic bacteria and methanogens. A speedy recovery is essential for an establishment of a feasible economical biogas productions. Yet, little is known regarding the organisms participating in the recovery. In this study the organisms involved in the recovery were studied using protein-stable isotope probing (Protein-SIP) and mapping this data onto a binned metagenome. Under acetate-accumulated simulating conditions a formation of 13C-labeled CO2 and CH4 was detected immediately after the addition of [U-13C]acetate, indicative of a high turnover rate of acetate. Several labeled peptides were detected in protein-SIP analysis. These 13C-labeled peptides were mapped onto a binned metagenome for improved taxanomical classification of the organisms involved. The results revealed that Methanosarcina and Methanoculleus were actively involved in acetate turnover, as were five subspecies of Clostridia and one Bacteroidetes. The organisms affiliating with Clostridia and Bacteroidetes all contained the FTFHS gene for formyltetrahydrofolate synthetase, a key enzyme for reductive acetogenesis; indicating that these organisms are possible syntrophic acetate-oxidizing bacteria (SAOB) that can facilitate acetate consumption via syntrophic acetate oxidation coupled with hydrogenotrophic methanogenesis (SAO-HM). This study represents the first study applying protein-SIP for analysis of complex biogas samples, a promising method for identifying key microorganisms involved in specific pathways.
HostingRepository	PRIDE
AnnounceDate	2016-05-11
AnnouncementXML	Submission_2016-05-11_06:54:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henrik Kjeldal
SpeciesList	scientific name: Methanoculleus sp. 10; NCBI TaxID: 430615;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-01 02:31:08	ID requested
⏵ 1	2016-05-11 06:54:18	announced

Mosb, æ, k F, Kjeldal H, Mulat DG, Albertsen M, Ward AJ, Feilberg A, Nielsen JL, Identification of syntrophic acetate-oxidizing bacteria in anaerobic digesters by combined protein-based stable isotope probing and metagenomics. ISME J, 10(10):2405-18(2016) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS/MS, Protein-Stable Isotope Probing, Anaerobic digesters, Biogas

Jeppe Lund Nielsen
contact affiliation	Professor in Environmental Microbiology and Biotechnology,Section for Biotechnology Department of Chemistry and Bioscience, Aalborg University, Denmark
contact email	jln@bio.aau.dk
lab head
Henrik Kjeldal
contact affiliation	Aalborg University
contact email	kjeldal@bio.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002996
     1. Label: PRIDE project
     2. Name: Identification of syntrophic acetate-oxidizing bacteria in anaerobic digesters