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PXD002979

PXD002979 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEquine tendon LC-MSMS, C4PR_LIV
Descriptionthe aim of this study was to isolate, characterise and compare the proteome of the IFM and fascicular matrix (FM) in the equine superficial digital flexor tendon (SDFT). We hypothesised that the proteomic profile would differ between the FM and the IFM, with greater complexity and faster turnover in the IFM. Regions of the SDFT were isolated using laser capture microdissection and the protein profile assessed using mass spectrometry. 246 proteins were identified in the IFM, and 141 were identified in the FM. 60 proteins showed a 2-fold difference in abundance between the IFM and FM, including greater abundance of collagen-III and cellular proteins in the IFM, and higher levels of collagens-I and XII, and fibromodulin in the FM. A greater number of neopeptides were identified in the IFM, indicating more rapid turnover than in the FM. There were few alterations in protein abundance with ageing, but the number of neopeptides decreased with age in the IFM specifically, suggesting a decrease in IFM turnover in aged individuals. These results support the hypothesis, showing distinct protein profiles and indicating more rapid turnover in the IFM than in the FM, which appears to decline with ageing in the IFM specifically. This may result in alterations in IFM mechanical properties in aged tendon, contributing to age-related tendon injury.
HostingRepositoryPRIDE
AnnounceDate2017-10-24
AnnouncementXMLSubmission_2017-10-24_00:52:00.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002979
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDeborah Simpson
SpeciesList scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationListOxidation; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-28 01:55:58ID requested
12016-02-08 09:02:55announced
22017-03-22 01:30:31announcedUpdated project metadata.
32017-10-24 00:52:01announcedUpdated project metadata.
Publication List
Thorpe CT, Peffers MJ, Simpson D, Halliwell E, Screen HR, Clegg PD, Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition. Sci Rep, 6():20455(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Equine, tendon, IFM, FM, LC-MSMS
Contact List
Dr ChavaunneThorpe
contact affiliationInstitute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK
contact emailc.thorpe@qmul.ac.uk
lab head
Deborah Simpson
contact affiliationUniversity of Liverpool
contact emaildsimpson@liv.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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