PXD002979 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Equine tendon LC-MSMS, C4PR_LIV |
Description | the aim of this study was to isolate, characterise and compare the proteome of the IFM and fascicular matrix (FM) in the equine superficial digital flexor tendon (SDFT). We hypothesised that the proteomic profile would differ between the FM and the IFM, with greater complexity and faster turnover in the IFM. Regions of the SDFT were isolated using laser capture microdissection and the protein profile assessed using mass spectrometry. 246 proteins were identified in the IFM, and 141 were identified in the FM. 60 proteins showed a 2-fold difference in abundance between the IFM and FM, including greater abundance of collagen-III and cellular proteins in the IFM, and higher levels of collagens-I and XII, and fibromodulin in the FM. A greater number of neopeptides were identified in the IFM, indicating more rapid turnover than in the FM. There were few alterations in protein abundance with ageing, but the number of neopeptides decreased with age in the IFM specifically, suggesting a decrease in IFM turnover in aged individuals. These results support the hypothesis, showing distinct protein profiles and indicating more rapid turnover in the IFM than in the FM, which appears to decline with ageing in the IFM specifically. This may result in alterations in IFM mechanical properties in aged tendon, contributing to age-related tendon injury. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_00:52:00.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002979 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Deborah Simpson |
SpeciesList | scientific name: Equus caballus (Horse); NCBI TaxID: 9796; |
ModificationList | Oxidation; Oxidation; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-28 01:55:58 | ID requested | |
1 | 2016-02-08 09:02:55 | announced | |
2 | 2017-03-22 01:30:31 | announced | Updated project metadata. |
⏵ 3 | 2017-10-24 00:52:01 | announced | Updated project metadata. |
Publication List
Thorpe CT, Peffers MJ, Simpson D, Halliwell E, Screen HR, Clegg PD, Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition. Sci Rep, 6():20455(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Equine, tendon, IFM, FM, LC-MSMS |
Contact List
Dr ChavaunneThorpe |
contact affiliation | Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK |
contact email | c.thorpe@qmul.ac.uk |
lab head | |
Deborah Simpson |
contact affiliation | University of Liverpool |
contact email | dsimpson@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002979
- Label: PRIDE project
- Name: Equine tendon LC-MSMS, C4PR_LIV