PXD002973

PXD002973 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Metabolic reprogramming in the airway epithelium of high risk individuals
Description	We investigated proteins identified by shotgun proteomics in cytologically normal airway epithelial cells from individuals at different levels of risk for lung cancer. We identified 2869 proteins in bronchial brushings from individuals at low, moderate or high risk for lung cancer. Pathway analysis revealed enrichment of carbohydrate metabolic pathways in high risk individuals. Differential expression of selected proteins was validated by parallel reaction monitoring mass spectrometry in separate individual bronchial brushings. Augmentation of glucose consumption and lactate production measured in human bronchial epithelial cell BEAS2B treated with cigarette smoke condensate and increased synthetic ability and reductive carboxylation revealed by metabolic flux analysis indicated profound metabolic reprogramming.
HostingRepository	MassIVE
AnnounceDate	2017-08-31
AnnouncementXML	Submission_2017-08-31_12:10:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robbert Slebos
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-25 05:16:23	ID requested
⏵ 1	2017-08-31 12:10:38	announced

Publication List

Rahman SMJ, Ji X, Zimmerman LJ, Li M, Harris BK, Hoeksema MD, Trenary IA, Zou Y, Qian J, Slebos RJ, Beane J, Spira A, Shyr Y, Eisenberg R, Liebler DC, Young JD, Massion PP, The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer. JCI Insight, 1(19):e88814(2016) [pubmed]

Rahman SMJ, Ji X, Zimmerman LJ, Li M, Harris BK, Hoeksema MD, Trenary IA, Zou Y, Qian J, Slebos RJ, Beane J, Spira A, Shyr Y, Eisenberg R, Liebler DC, Young JD, Massion PP, The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer. JCI Insight, 1(19):e88814(2016) [pubmed]

Keyword List

submitter keyword: airway epithelium, cigarette smoke, metabolic reprogramming, carbohydrate metabolism

submitter keyword: airway epithelium, cigarette smoke, metabolic reprogramming, carbohydrate metabolism

Contact List

Pierre Massion
lab head
Robbert Slebos
contact affiliation	Moffitt
contact email	robbert.slebos@moffitt.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079331

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079331