PXD002972

PXD002972 is an original dataset announced via ProteomeXchange.

Title	Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast
Description	In eukaryotes, the conjugation of proteins to the small ubiquitin-like modifier SUMO regulates numerous cellular functions. A proportion of SUMO conjugates are targeted for degradation by SUMO-targeted ubiquitin ligases (STUbLs) and it has been proposed that the ubiquitin-selective chaperone Cdc48/p97-Ufd1-Npl4 facilitates this process. However, the extent to which the two pathways overlap, and how the substrates are selected, remains unknown. Here, we address these questions in fission yeast through proteome-wide analyses of SUMO modification sites. We identify over a thousand sumoylated lysines in a total of 468 proteins and quantify changes occurring in the SUMO modification status when the STUbL or Ufd1 pathways are compromised by mutations. The data suggest the coordinated processing of several classes of SUMO conjugates, many dynamically associated with centromeres or telomeres. They provide new insights into subnuclear organization and chromosome biology, and, altogether, constitute an extensive resource for the molecular characterization of SUMO function and dynamics.
HostingRepository	PRIDE
AnnounceDate	2015-10-30
AnnouncementXML	Submission_2015-11-09_01:51:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Triin Tammsalu
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	acetylated residue; phosphorylated residue; iodoacetamide derivatized residue; ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-25 01:30:59	ID requested
1	2015-10-30 09:44:32	announced
⏵ 2	2015-11-09 01:51:59	announced	Updated publication reference for PubMed record(s): 26537787.

K, ø, hler JB, Tammsalu T, J, ø, rgensen MM, Steen N, Hay RT, Thon G, Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast. Nat Commun, 6():8827(2015) [pubmed]

curator keyword: Biological

submitter keyword: S. pombe, LC-MSMS, SUMO modification sites, STUbL, Cdc48-Ufd1-Npl4

Genevieve Thon
contact affiliation	University of Copenhagen
contact email	gen@bio.ku.dk
lab head
Triin Tammsalu
contact affiliation	University of Dundee
contact email	t.tammsalu@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002972

PRIDE project URI

• PRIDE
  1. PXD002972
     1. Label: PRIDE project
     2. Name: Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast