PXD002966

PXD002966 is an original dataset announced via ProteomeXchange.

Title	OXIPEP: mass spectrometry strategy to characterize protein carbonylation.
Description	Here we describe a peptide-centric approach (OXIPEP), where carbonylated residues within proteins are derivatized with biotin-hydrazide, enriched and identified by mass spectrometry. The strength of the method lies in an improved protocol for elution of biotinylated peptides from monomeric avidin resin using hot water (95C) and increased sensitivity achieved by reduction of sample losses during sample preparation and chromatographic steps. For the first time advanced bioinformatics analysis utilising diagnostic biotin fragment ions is used to facilitate semi-automatic in silico evaluation of biotin labelling and to increase the reliability of carbonyl peptide identification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:25:52.553.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adelina Rogowska-Wrzesinska
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-24 03:58:15	ID requested
1	2018-10-18 11:29:10	announced
⏵ 2	2024-10-22 04:26:00	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2016.12.019;

Havelund JF, Wojdyla K, Davies MJ, Jensen ON, M, ø, ller IM, Rogowska-Wrzesinska A, A biotin enrichment strategy identifies novel carbonylated amino acids in proteins from human plasma. J Proteomics, 156():40-51(2017) [pubmed]

curator keyword: Technical

submitter keyword: protein carbonylation, bovine serum albumine, human plasma, biotin-hydrazide

Ole N. Jensen
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
contact email	jenseno@bmb.sdu.dk
lab head
Adelina Rogowska-Wrzesinska
contact affiliation	University of Southern Denmark
contact email	adelinar@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD002966

PRIDE project URI

• PRIDE
  1. PXD002966
     1. Label: PRIDE project
     2. Name: OXIPEP: mass spectrometry strategy to characterize protein carbonylation.