PXD002965 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches |
Description | Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Data presented here allowed a proteomic characterization of the interactome of RACK1 in drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent (DDA) and Data-Independent Acquisition (DIA, SWATH). This study is a 2-factor experiment, where control samples (RACK1 overexpressed with a HA tag) were compared to IP samples (RACK1 overexpressed with a FLAG tag), in either mock or infected cells. Each of these 6 conditions were performed in biological triplicates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:26:25.921.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lauriane Kuhn |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-24 00:43:28 | ID requested | |
1 | 2017-05-29 05:29:57 | announced | |
⏵ 2 | 2024-10-22 04:26:28 | announced | 2024-10-22: Updated project metadata. |
Publication List
Kuhn L, Majzoub K, Einhorn E, Chicher J, Pompon J, Imler JL, Hammann P, Meignin C, Using SWATH-MS. G3 (Bethesda), 7(7):2249-2258(2017) [pubmed] |
10.1534/g3.117.042564; |
Keyword List
curator keyword: Biological |
submitter keyword: Affinity purification, RNA interference,RACK1, interactomics, Drosophila |
Contact List
Philippe Hammann |
contact affiliation | CNRS, IBMC, FRC1589, Proteomics Core Facility |
contact email | p.hammann@ibmc-cnrs.unistra.fr |
lab head | |
Lauriane Kuhn |
contact affiliation | CNRS, IBMC, FRC1589 |
contact email | l.kuhn@ibmc-cnrs.unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD002965 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002965
- Label: PRIDE project
- Name: RACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches