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PXD002963

PXD002963 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profile of coffee embryogenic cell suspensions
DescriptionCoffee is one of the most important commodities cultivated worldwide and has great economic impact in producing countries. Although 130 different species belonging to the coffea gender have been described, only two of them are commercially exploited: Coffea arabica and Coffea canephora. C. arabica is responsible for 61% of the world production (Van der Vossen et al., 2015). However, due to the narrow genetic back ground, classical genetic breeding is time consuming and takes around 30 years (Santana-Buzzy et al., 2007; Hendre et al., 2014). Several genetic engineering and biotechnological tools have been successfully applied in coffee breeding. Somatic embryogenesis (SE) is a process in which new viable embryos are produced from somatic tissues. It is one of the most promising production processes (Santana-Buzzy et al, 2007; Marsoni et al., 2008). A better understanding of the molecular basis related to somatic embryogenesis will give insight into the process of embryo formation and totipotency and will allow the development of new in vitro culture strategies for the propagation and genetic manipulation of elite cultivars (Marsoni et al., 2008). High throughput proteomics in coffee is limited so far to 2D gel based proteomics techniques. Although really useful and the most common technique for plants, 2DE is limited in throughput and a gel free technique allow to go a step further (Carpentier & America, 2014; Vanhove et al., 2015). To improve the knowledge about somatic embryogenesis, we present the first high throughput proteome profile (1051 confident protein identifications) of coffee embryogenic cell suspensions developed from leaves of Coffea arabica cultivar CatuaĆ­.
HostingRepositoryPRIDE
AnnounceDate2016-02-03
AnnouncementXMLSubmission_2016-03-29_08:53:20.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002963
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterNadia Campos
SpeciesList scientific name: Coffea arabica (Arabian coffee); NCBI TaxID: 13443;
ModificationListOxidation; Glu->pyro-Glu; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-23 06:40:37ID requested
12016-02-03 08:35:30announced
22016-03-29 08:53:21announcedUpdated publication reference for PubMed record(s): 27001127.
Publication List
Campos NA, Paiva LV, Panis B, Carpentier SC, The proteome profile of embryogenic cell suspensions of Coffea arabica L. Proteomics, 16(6):1001-5(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Coffea arabica, somatic embryogenesis, totipotency, gel free proteomics
Contact List
Sebastien Carpentier
contact affiliationDepartament of Byossistems, KULeuven, Leuven 3000, Belgium
contact emailSebastien.Carpentier@kuleuven.be
lab head
Nadia Campos
contact affiliationKULeuven
contact emailnadialvesnep@hotmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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