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PXD002953

PXD002953 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecreted Proteins of Escherichia coli Serotypes O157:H7 and O104:H4 Cultured in Minimal Medium
DescriptionEscherichia coli is a diverse species of bacteria. Some isolates of E. coli can cause hemorrhagic uremic syndrome in humans through consumption of contaminated food. We hypothesize that secreted and surface-associated proteins in E. coli may contribute to bacterial persistence in food products and to pathogenicity in humans. Here we report the proteomic analyses of secretomes from two serotypes of E. coli, O157:H7 and O104:H4, that cause human foodborne illness. The secreted proteins were isolated from the liquid cultures and analyzed by liquid chromatography-tandem mass spectrometry. We report 57 secreted proteins for O157:H7 and 67 for O104:H4 with increased abundance in minimal medium. As signature proteins, O157:H7 expressed intimin and translocated intimin receptor. By contrast, O104:H4 expressed enteroaggregative fimbriae, serine protease autotransporters, Shiga toxin, and beta-lactamase. The E. coli O104:H4 released more cytosolic proteins into the culture media than the O157:H7 serotype, indicating that it might be a leaky strain. The differential abundance of two transpeptidases YbiS and YnhG, along with other lipoproteins in O157:H7 indicates that this serotype secretes several peptidol-lipoprotein components of biofilm. In addition, we observed differential abundance of several ABC transporters, proteases and lipoproteins, which give each strain a unique survival strategy and drug resistance. The identification of secreted proteins will enable us understand better how these bacteria persist in food products and cause human disease.
HostingRepositoryMassIVE
AnnounceDate2015-09-21
AnnouncementXMLSubmission_2015-09-21_13:18:56.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitter
SpeciesList scientific name: Escherichia coli;
ModificationListCarbamidomethyl
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-21 12:51:49ID requested
12015-09-21 13:18:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Escherichia coli O157:H7, O104:H4, secretomes, mass spectrometry, MudPIT
Contact List
Nazrul Islam
lab head
contact affiliation
contact emailnazrul@umd.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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