PXD002944

PXD002944 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of testis from infertile protein C inhibitor deficient mice reveals predominant changes in serpin processing and prostaglandin metabolism
Description	Serine protease inhibitors have therapeutic potential in a variety of pathogenic processes, ranging from thrombosis, altered immune response to liver cirrhosis. To investigate the physiological effects of protein C inhibitor (PCI, serpinA5) this gene was inactivated in a mouse model with the resulting phenotype of male infertility. In the actual report 2D-DIGE analysis was utilized to investigate the molecular mechanisms for PCI in male reproduction. Comparing the testes proteome of three PCI knockout-mice with the three wild-types- demonstrated similar pattern with the exception of a massive upregulation of prostaglandin reductase 1 (10-fold; P < 0.002) and complete shifts in the molecular weights of serpinA1C and serpinA3K. All these PCI-dependent proteome changes were immunologically verified. Unbiased proteome analysis indicated that the inactivation of serpinA5 strongly influenced both the protein species pattern of other A-clade serpins as well as the prostaglandin metabolism in the testes.
HostingRepository	PRIDE
AnnounceDate	2015-12-07
AnnouncementXML	Submission_2015-12-07_01:25:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002944
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Goran Mitulović
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Ammonia-loss: -17.026549; Oxidation: 15.994915; Phospho: 79.966331; Carbamidomethyl: 57.021464; Acetyl: 42.010565; Glu->pyro-Glu: -18.010565; Gln->pyro-Glu: -17.026549
Instrument	LTQ Velos; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-21 01:23:20	ID requested
⏵ 1	2015-12-07 01:25:51	announced

Yang H, Wahlm, ü, ller FC, Uhrin P, Baumgartner R, Mitulovic G, Sarg B, Geiger M, Zellner M, Proteome analysis of testis from infertile protein C inhibitor-deficient mice reveals novel changes in serpin processing and prostaglandin metabolism. Electrophoresis, 36(21-22):2837-2840(2015) [pubmed]

curator keyword: Biological

submitter keyword: mouse, proteomics, serpin,

Maria Zellner
contact affiliation	Maria Zellner, Institute of Physiology, Center of Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria
contact email	maria.zellner@meduniwien.ac.at
lab head
Goran Mitulović
contact affiliation	Medizinische Universität Wien
contact email	goran.mitulovic@meduniwien.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002944
     1. Label: PRIDE project
     2. Name: Proteome analysis of testis from infertile protein C inhibitor deficient mice reveals predominant changes in serpin processing and prostaglandin metabolism