PXD002941

PXD002941 is an original dataset announced via ProteomeXchange.

Title	Novel N-terminal and lysine methyltransferases that target translation elongation factor 1A in yeast and human
Description	Eukaryotic elongation factor 1A (eEF1A) is an essential, highly methylated protein that facilitates translational elongation by delivering aminoacyl-tRNAs to ribosomes. Here we report a new eukaryotic protein N-terminal methyltransferase, Saccharomyces cerevisiae YLR285W, which methylates eEF1A at a previously undescribed high-stoichiometry N-terminal site and the adjacent lysine. Deletion of YLR285W resulted in the loss of N-terminal and lysine methylation in vivo, whereas overexpression of YLR285W resulted in an increase of methylation at these sites. This was confirmed by in vitro methylation of eEF1A by recombinant YLR285W. Accordingly, we name YLR285W as elongation factor methyltransferase 7 (Efm7). This enzyme is a new type of eukaryotic N-terminal methyltransferase as, unlike the three other known eukaryotic N-terminal methyltransferases, its substrate does not have an N-terminal [A/P/S]-P-K motif. We show that the N-terminal methylation of eEF1A is also present in human; this conservation over a large evolutionary distance suggests it to be of functional importance. This study also reports that the trimethylation of K79 in eEF1A is conserved from yeast to human. The methyltransferase responsible for K79 methylation of human eEF1A is shown to be N6AMT2, previously documented as a putative N(6)-adenine-specific DNA methyltransferase. It is the direct ortholog of the recently described yeast Efm5 and we show that Efm5 and N6AMT2 can methylate eEF1A from either species in vitro. We therefore rename N6AMT2 as eEF1A-KMT1. Including the present work, yeast eEF1A is now documented to be methylated by five different methyltransferases, making it one of the few eukaryotic proteins to be extensively methylated by independent enzymes. This implies more extensive regulation of eEF1A by this post-translational modification than previously appreciated.
HostingRepository	PRIDE
AnnounceDate	2015-11-10
AnnouncementXML	Submission_2015-11-10_03:36:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joshua Hamey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; methylated residue; monomethylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-18 03:00:44	ID requested
⏵ 1	2015-11-10 03:36:07	announced

Hamey JJ, Winter DL, Yagoub D, Overall CM, Hart-Smith G, Wilkins MR, Novel N-terminal and Lysine Methyltransferases That Target Translation Elongation Factor 1A in Yeast and Human. Mol Cell Proteomics, 15(1):164-76(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Methylation, methyltransferases, N-terminus, lysine, post-translational modifications

Marc Ronald Wilkins
contact affiliation	Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, New South Wales, 2052, Australia
contact email	m.wilkins@unsw.edu.au
lab head
Joshua Hamey
contact affiliation	University of New South Wales
contact email	joshua.hamey@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002941
     1. Label: PRIDE project
     2. Name: Novel N-terminal and lysine methyltransferases that target translation elongation factor 1A in yeast and human