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PXD002939

PXD002939 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential gene expression in Leishmania [SPP.] promastigotes. Leishmania chagasi
DescriptionLeishmania chagasi is the causative agent of zoonotic visceral leishmaniasis in Brazil, being domestic and stray dogs the main reservoirs. The development of the parasite involves two stages. The promastigote is extracellular and develops within the sand fly gut. The amastigote survives inside the harsh environment of the phagolysosome of mammalian host phagocytes, where pH is acidic, temperature higher than in the sand fly vector and hydrolytic enzymes act. In addition, the host phagocyte displays the nitric oxide defense mechanism against the amastigote. Promastigotes are also able to withstand NO even when they develop within the sand fly gut. This can be explained with the pre-adaptative hypothesis, which has been supported by us and others elsewhere and consists of preparation of promastigotes in advance for development towards the amastigote stage. For this reason, the comparison of NO-resistant and sensitive promastigotes is valuable. The two-dimension electrophoresis-mass spectrometry (2DE-MS/MS) approach has been performed to study differential protein abundance comparing L. chagasi NO-sensitive and resistant promastigotes. This analysis has revealed differential expression of genes directly and indirectly involved in NO-resistance, highlighting up-regulation of the glucose-6-phosphate dehydrogenase (G6PD) in NO-resistant promastigotes and down-regulation of the glutathione peroxidase (GPX) and the arginase (ARG) in NO-sensitive ones. These data are a starting point in the search of vaccine candidates and/or drug targets.
HostingRepositoryPRIDE
AnnounceDate2016-04-01
AnnouncementXMLSubmission_2016-03-31_16:10:12.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002939
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJ. Alberto Medina-Aunon
SpeciesList scientific name: Leishmania chagasi; NCBI TaxID: 44271;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
Instrument4800 Proteomics Analyzer
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-18 02:11:09ID requested
12016-03-31 16:10:13announced
Publication List
Alcolea PJ, Alonso A, Garc, í, a-Tabares F, Mena MDC, Ciordia S, Larraga V, Proteome profiling of the growth phases of Leishmania pifanoi promastigotes in axenic culture reveals differential abundance of immunostimulatory proteins. Acta Trop, 158():240-247(2016) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: 2D-E, MALDI, Leishmania, Differential gene expression
Contact List
Vicente Larraga
contact affiliationVaccine and Gene Expresion group. Molecular Microbiology and Infection Biology. Centro de Investigaciones Biológicas - CSIC. Madrid, Spain
contact emailvlarraga@cib.csic.es
lab head
J. Alberto Medina-Aunon
contact affiliationProteomics - Bioinformatics
contact emailjamedina@proteored.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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