PXD002939 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differential gene expression in Leishmania [SPP.] promastigotes. Leishmania chagasi |
Description | Leishmania chagasi is the causative agent of zoonotic visceral leishmaniasis in Brazil, being domestic and stray dogs the main reservoirs. The development of the parasite involves two stages. The promastigote is extracellular and develops within the sand fly gut. The amastigote survives inside the harsh environment of the phagolysosome of mammalian host phagocytes, where pH is acidic, temperature higher than in the sand fly vector and hydrolytic enzymes act. In addition, the host phagocyte displays the nitric oxide defense mechanism against the amastigote. Promastigotes are also able to withstand NO even when they develop within the sand fly gut. This can be explained with the pre-adaptative hypothesis, which has been supported by us and others elsewhere and consists of preparation of promastigotes in advance for development towards the amastigote stage. For this reason, the comparison of NO-resistant and sensitive promastigotes is valuable. The two-dimension electrophoresis-mass spectrometry (2DE-MS/MS) approach has been performed to study differential protein abundance comparing L. chagasi NO-sensitive and resistant promastigotes. This analysis has revealed differential expression of genes directly and indirectly involved in NO-resistance, highlighting up-regulation of the glucose-6-phosphate dehydrogenase (G6PD) in NO-resistant promastigotes and down-regulation of the glutathione peroxidase (GPX) and the arginase (ARG) in NO-sensitive ones. These data are a starting point in the search of vaccine candidates and/or drug targets. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-01 |
AnnouncementXML | Submission_2016-03-31_16:10:12.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002939 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | J. Alberto Medina-Aunon |
SpeciesList | scientific name: Leishmania chagasi; NCBI TaxID: 44271; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | 4800 Proteomics Analyzer |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-18 02:11:09 | ID requested | |
⏵ 1 | 2016-03-31 16:10:13 | announced | |
Publication List
Alcolea PJ, Alonso A, Garc, í, a-Tabares F, Mena MDC, Ciordia S, Larraga V, Proteome profiling of the growth phases of Leishmania pifanoi promastigotes in axenic culture reveals differential abundance of immunostimulatory proteins. Acta Trop, 158():240-247(2016) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: 2D-E, MALDI, Leishmania, Differential gene expression |
Contact List
Vicente Larraga |
contact affiliation | Vaccine and Gene Expresion group. Molecular Microbiology and Infection Biology. Centro de Investigaciones Biológicas - CSIC. Madrid, Spain |
contact email | vlarraga@cib.csic.es |
lab head | |
J. Alberto Medina-Aunon |
contact affiliation | Proteomics - Bioinformatics |
contact email | jamedina@proteored.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD002939 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002939
- Label: PRIDE project
- Name: Differential gene expression in Leishmania [SPP.] promastigotes. Leishmania chagasi