PXD002936

PXD002936 is an original dataset announced via ProteomeXchange.

Title	The Proteome of the Infectious Bronchitis Virus Beau-R Virion
Description	Infectious bronchitis is a highly contagious respiratory disease of poultry caused by the coronavius infectious bronchitis virus (IBV). Until recently is was thought that coronavirus virions were composed of the structural proteins nucleocapsid, envelope, spike and membrane proteins, but investigations of TGEV and SARS-CoV have shown the proteome of coronavirus virions also includes viral non-structural and group specific proteins as well as host cell proteins. To study the proteome of IBV virions, virus was grown in embryonated chicken eggs and purified by sucrose gradient ultracentrifugation. Purified virus was analysed using sensitive gel-free proteomic techniques to determine the proteome of IBV. Analysis of three preparations of purified IBV yielded a list of 39 proteins commonly associated with the IBV virion. Three of these proteins were the viral structural proteins spike, membrane and nucleocapsid, but none of the viral non-strucutral or groups specific proteins could be identified. The other 35 proteins commonly associated to the IBV virion were all found to be host cell proteins. These proteins were classified into 12 categories using pantherdb (pantherdb.org). These proteins were involved in a diverse range of functions such as cytoskeletal proteins, nucleic acid binding proteins and chaperone proteins. Some of these proteins were unique to this study, whilst others were found to be orthologous to proteins identified in the SARS-CoV protein, and indeed some were also identified in association with virions from a number of other RNA and DNA viruses.
HostingRepository	PRIDE
AnnounceDate	2016-02-15
AnnouncementXML	Submission_2016-06-06_04:49:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Xia
SpeciesList	scientific name: Avian infectious bronchitis virus (strain Beaudette CK) (IBV); NCBI TaxID: 160235;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-17 04:05:19	ID requested
1	2016-02-15 07:47:24	announced
⏵ 2	2016-06-06 04:49:25	announced	Updated publication reference for PubMed record(s): 27257648.

Dent SD, Xia D, Wastling JM, Neuman BW, Britton P, Maier HJ, The proteome of the infectious bronchitis virus Beau-R virion. J Gen Virol, 96(12):3499-3506(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Infectious Bronchitis Virus, Beau-R Virion

Jonathan M Wastling
contact affiliation	Faculty of Natural Sciences, Keele University, UK
contact email	j.wastling@keele.ac.uk
lab head
Dong Xia
contact affiliation	Infection Biology
contact email	d.xia@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002936

PRIDE project URI

• PRIDE
  1. PXD002936
     1. Label: PRIDE project
     2. Name: The Proteome of the Infectious Bronchitis Virus Beau-R Virion