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PXD002934

PXD002934 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell surface proteomic map of HIV infection reveals antagonism of amino acid metabolism by Vpu and Nef
DescriptionCritical cell surface immunoreceptors downregulated by HIV have previously been identified using non-systematic, candidate approaches. To gain a comprehensive, unbiased overview of how HIV infection remodels the T-cell surface, we took a distinct, systems-level, quantitative proteomic approach. HIV downregulated >100 plasma membrane proteins, many without characterised roles in the immune system. An exclusive group of host factors were targeted by the viral accessory proteins Vpu or Nef, including the amino acid transporter SNAT1 and the serine carriers SERINC3/5. We focussed on SNAT1, a novel, ß- TrCP-dependent Vpu substrate. Antagonism of SNAT1 emerges in Vpu variants from the lineage of SIVcpz/HIV-1 viruses responsible for pandemic AIDS. We found marked SNAT1 induction in activated primary human CD4+ T-cells, and used Consumption and Release (CoRe) metabolomics to identify alanine as an endogenous SNAT1 substrate required for T- cell mitogenesis. Downregulation of SNAT1 therefore defines a novel paradigm of viral interference with immunometabolism.
HostingRepositoryPRIDE
AnnounceDate2015-11-09
AnnouncementXMLSubmission_2015-11-09_08:06:18.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYagnesh Umrania
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-17 02:51:55ID requested
12015-11-09 08:06:19announced
Publication List
Matheson NJ, Sumner J, Wals K, Rapiteanu R, Weekes MP, Vigan R, Weinelt J, Schindler M, Antrobus R, Costa AS, Frezza C, Clish CB, Neil SJ, Lehner PJ, Cell Surface Proteomic Map of HIV Infection Reveals Antagonism of Amino Acid Metabolism by Vpu and Nef. Cell Host Microbe, 18(4):409-23(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: HIV, Virus infection, Cell surface proteome
Contact List
Paul Lehner
contact affiliationUniversity of Cambridge
contact emailpjl30@cam.ac.uk
lab head
Yagnesh Umrania
contact affiliationUniversity of Cambridge
contact emailyu206@cam.ac.uk
dataset submitter
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Dataset FTP location
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