PXD002933

PXD002933 is an original dataset announced via ProteomeXchange.

Title	The E. coli type III secreted effectors NleB and EspL interact with the microtubule associated protein ensconsin to disrupt vesicle trafficking, part 1
Description	Enteropathogenic and enterohaemorrhagic E. coli (EPEC and EHEC) translocate a set of type III effector proteins into host cells that are critical for bacterial virulence. These effectors subvert normal host pathways by interacting with a variety of targets within the cell, but the binding partners and mechanism of action of the majority of effectors are not understood. We identified the microtubule associated protein, ensconsin, as a novel target for two EPEC/EHEC effectors. We found that the secreted effectors NleB and EspL bind host ensconsin and work synergistically to paralyze kinesin-based intracellular vesicular transport. Our findings demonstrate that EPEC/EHEC encode multiple effectors that control intracellular vesicle movement and suggest a simple strategy for broad-based immobilization of host cells by pathogens.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:12:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leonard Foster
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-17 00:57:07	ID requested
1	2016-12-21 23:47:54	announced
⏵ 2	2017-10-24 03:12:08	announced	Updated project metadata.

Law RJ, Law HT, Scurll JM, Scholz R, Santos AS, Shames SR, Deng W, Croxen MA, Li Y, de Hoog CL, van der Heijden J, Foster LJ, Guttman JA, Finlay BB, Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors. J Proteome Res, 15(5):1613-22(2016) [pubmed]

curator keyword: Biological

submitter keyword: Human, E. coli, host-pathogen, effector

Leonard James Foster
contact affiliation	Centre for High-Throughput Biology, University of British Columbia
contact email	foster@chibi.ubc.ca
lab head
Leonard Foster
contact affiliation	Centre for High Throughput Biology
contact email	ljfoster@interchange.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD002933

PRIDE project URI

• PRIDE
  1. PXD002933
     1. Label: PRIDE project
     2. Name: The E. coli type III secreted effectors NleB and EspL interact with the microtubule associated protein ensconsin to disrupt vesicle trafficking, part 1