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PXD002929

PXD002929 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
DescriptionWe present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility during chromatographic separation. We show this efficiently detects both integral membrane and membrane-associated protein complexes, in addition to soluble complexes, allowing identification and analysis of complexes not previously accessible in native extracts. Using this approach, we demonstrate how these data may be applied to the analysis of membrane protein complexes and to discriminate between interactions of different protein isoforms. The entire proteome-wide dataset of in vivo crosslinked protein complexes from U2OS cells is available via a searchable online database (www.peptracker.com/epd).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:37:19.088.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVackar Afzal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList(R)-5-oxo-1; phosphorylated residue; monohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-17 00:03:48ID requested
12022-05-30 09:19:45announced
22024-10-22 05:37:19announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.o115.055467;
Larance M, Kirkwood KJ, Tinti M, Brenes Murillo A, Ferguson MA, Lamond AI, Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling. Mol Cell Proteomics, 15(7):2476-90(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Crosslinking, Protein-Protein Interactions
Contact List
Angus I Lamond
contact affiliationCentre for Gene Regulation and Expression, University of Dundee
contact emaila.i.lamond@dundee.ac.uk
lab head
Vackar Afzal
contact affiliationSchool of Life Sciences
contact emailv.z.afzal@dundee.ac.uk
dataset submitter
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Dataset FTP location
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