PXD002929 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling |
Description | We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility during chromatographic separation. We show this efficiently detects both integral membrane and membrane-associated protein complexes, in addition to soluble complexes, allowing identification and analysis of complexes not previously accessible in native extracts. Using this approach, we demonstrate how these data may be applied to the analysis of membrane protein complexes and to discriminate between interactions of different protein isoforms. The entire proteome-wide dataset of in vivo crosslinked protein complexes from U2OS cells is available via a searchable online database (www.peptracker.com/epd). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:37:19.088.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vackar Afzal |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | (R)-5-oxo-1; phosphorylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-17 00:03:48 | ID requested | |
1 | 2022-05-30 09:19:45 | announced | |
⏵ 2 | 2024-10-22 05:37:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.o115.055467; |
Larance M, Kirkwood KJ, Tinti M, Brenes Murillo A, Ferguson MA, Lamond AI, Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling. Mol Cell Proteomics, 15(7):2476-90(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Crosslinking, Protein-Protein Interactions |
Contact List
Angus I Lamond |
contact affiliation | Centre for Gene Regulation and Expression, University of Dundee |
contact email | a.i.lamond@dundee.ac.uk |
lab head | |
Vackar Afzal |
contact affiliation | School of Life Sciences |
contact email | v.z.afzal@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002929
- Label: PRIDE project
- Name: Global Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling