PXD002928

PXD002928 is an original dataset announced via ProteomeXchange.

Title	Mammalian Target of Rapamycin shapes the cytotoxic T cell proteome
Description	High resolution mass spectrometry maps the CD8 cytotoxic cell (CTL) proteome and the impact of mammalian target of rapamycin complex 1 (mTORC1) on CTL. We show mTORC1 selectively represses and promotes expression of a subset (10%) of CTL proteins including key CTL effector and adhesion molecules and adaptor proteins. mTORC1 also controls flux through a selective subset of metabolic pathways but is not an on/off switch for CTL metabolism. Proteomic data highlighted the potential for mTORC1 negative control of phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P3) production in CTL. Further work revealed that mTORC1 represses PI(3,4,5)P3 production and controls the mTORC2 requirement for activation of the serine/threonine kinase AKT. Unbiased proteomic analysis thus provides a comprehensive understanding of CTL identity and mTORC1 control of CTL function.
HostingRepository	PRIDE
AnnounceDate	2015-10-13
AnnouncementXML	Submission_2015-11-11_02:15:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Hukelmann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deamidated residue; acetylated residue; iodoacetamide derivatized residue; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-17 00:00:13	ID requested
1	2015-10-13 07:24:50	announced
⏵ 2	2015-11-11 02:15:24	announced	Updated publication reference for PubMed record(s): 26551880.

Hukelmann JL, Anderson KE, Sinclair LV, Grzes KM, Murillo AB, Hawkins PT, Stephens LR, Lamond AI, Cantrell DA, The cytotoxic T cell proteome and its shaping by the kinase mTOR. Nat Immunol, 17(1):104-12(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: mTOR, lymphocytes, proteomics

Doreen Ann Cantrell
contact affiliation	Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK
contact email	d.a.cantrell@dundee.ac.uk
lab head
Jens Hukelmann
contact affiliation	University of Dundee
contact email	jhukelmann@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002928

PRIDE project URI

• PRIDE
  1. PXD002928
     1. Label: PRIDE project
     2. Name: Mammalian Target of Rapamycin shapes the cytotoxic T cell proteome