PXD002927

PXD002927 is an original dataset announced via ProteomeXchange.

Title	Phosphoprotein network analysis of white adipose tissues unveils deregulated pathways in response to high-fat diet
Description	Despite wide efforts in the last decade, signaling aberrations associated with obesity remain enigmatic. Here, we carried out phosphoproteomic analysis of mouse white adipose tissues (WAT) upon low-fat diet (LFD) and high-fat diet (HFD) to dissect underlying molecular mechanisms of obesity. Of the 7696 phosphopeptides quantified, 191 proteins including various insulin-responsive proteins and metabolic enzymes functioning in lipid homeostasis, exhibited differential phosphorylation with high-fat feeding. Kinase predictions and integrated network analysis identified several deregulated kinase signaling pathways, and suggested possibilities of HFD-induced transcriptional rewiring. Further, functional validation of a novel HFD-responsive site on cytoplasmic acetyl-coA forming ACSS2 (S263) suggested that the phosphorylation is important in regulating insulin signaling and maintaining triglyceride levels. This study represents one of the first comprehensive phosphoproteome data in mouse obesity models, and describes a systems-level approach for identifying deregulated molecular events and potential therapeutic targets in the context of high-fat feeding and adipocyte perturbation.
HostingRepository	PRIDE
AnnounceDate	2016-05-20
AnnouncementXML	Submission_2016-05-20_00:34:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hyungwon Choi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-16 23:51:34	ID requested
⏵ 1	2016-05-20 00:34:54	announced

Shaik AA, Qiu B, Wee S, Choi H, Gunaratne J, Tergaonkar V, Phosphoprotein network analysis of white adipose tissues unveils deregulated pathways in response to high-fat diet. Sci Rep, 6():25844(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Mouse Phosphoproteome, Diet

Jayantha Gunaratne
contact affiliation	Institute of Molecular and Cell Biology, ASTAR
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Hyungwon Choi
contact affiliation	National University of Singapore
contact email	hyung_won_choi@nuhs.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD002927

PRIDE project URI

• PRIDE
  1. PXD002927
     1. Label: PRIDE project
     2. Name: Phosphoprotein network analysis of white adipose tissues unveils deregulated pathways in response to high-fat diet