PXD002924 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nucleocytoplasmic O-Man glycoproteome |
Description | Dynamic cycling of N-Acetylglucosamine (GlcNAc) on serine (Ser) and threonine (Thr) residues (O-GlcNAcylation) is an essential process in all eukaryotic cells except yeast, e.g Saccharomyces cerevisiae. O-GlcNAcylation modulates signaling and cellular processes in an intricate interplay with protein phosphorylation, and serves as a key sensor of nutrients by linking the hexosamine biosynthetic pathway (HBP) to cellular signaling. A longstanding conundrum has been how yeast survives without O-GlcNAcylation in light of its similar phosphorylation signaling system. We previously developed a sensitive lectin enrichment and mass spectrometry workflow for identification of the human O-Mannose (O-Man) glycoproteome, and used this to identify a pleothora of O-linked mannose glycoproteins in human cell lines including the large family of cadherins and protocadherins. Here, we applied the workflow to yeast with the aim to characterize the yeast O-Man glycoproteome, and doing so we discovered hitherto unknown O-Man glycosites on nuclear, cytoplasmic and mitochondrial proteins in Saccharomyces cerevisiae. The type of nucleocytoplasmic proteins and the localization of identified O-Man residues mirrors that of the O-GlcNAc glycoproteome in other eukaryotic cells, indicating that the two different types of O-glycosylations serve the same important biological functions. The discovery opens for exploration of the enzyme machinery that is predicted to regulate the discovered nucleocytoplasmic O-Man glycosylation. It is likely that manipulation of this type of O-Man glycosylation will have wide applications for yeast bioprocessing. |
HostingRepository | PRIDE |
AnnounceDate | 2015-12-08 |
AnnouncementXML | Submission_2015-12-10_02:45:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adnan Halim |
SpeciesList | scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | hexosylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-16 06:37:35 | ID requested | |
1 | 2015-12-08 07:35:19 | announced | |
⏵ 2 | 2015-12-10 02:45:37 | announced | Updated publication reference for PubMed record(s): 26644575. |
Publication List
Halim A, Larsen IS, Neubert P, Joshi HJ, Petersen BL, Vakhrushev SY, Strahl S, Clausen H, Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast. Proc Natl Acad Sci U S A, 112(51):15648-53(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Saccharomyces cerevisiae, O-glycoproteomics |
Contact List
Henrik Clausen |
contact affiliation | Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark |
contact email | hclau@sund.ku.dk |
lab head | |
Adnan Halim |
contact affiliation | University of Copenhagen |
contact email | halim@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002924
- Label: PRIDE project
- Name: Nucleocytoplasmic O-Man glycoproteome