PXD002915

PXD002915 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteome analysis reveals the central role of macrophage lysosomes in regulating immune responses
Description	Phagocytosis and autophagy in macrophages have been shown to be essential to both innate and adaptive immunity. Lysosomes are the main catabolic subcellular organelles responsible for degradation and recycling of both extracellular and intracellular material, which are the final steps in phagocytosis and autophagy. Lysosomes are critical for the correct function of macrophages; however, the molecular mechanisms underlying lysosomal functions after infection remain to be fully clarified. In this study, we conducted a quantitative proteomics analysis of the changes in constitution and glycosylation of proteins in lysosomes derived from murine RAW 264.7 macrophage cells treated with different types of pathogens comprising examples of bacteria (Listeria monocytogenes, L. m), DNA viruses (Herpes simplex virus type-1, HSV-1) and RNA viruses (vesicular stomatitis virus, VSV). In total, 3,704 lysosome-related proteins and 264 glycosylated proteins were identified. Bioinformatic analysis showed lysosomes initiated distinct pathways according to the type of pathogens and function via integrated signaling of the innate and adaptive immune systems. Fluctuations in the glycosylation of several lysosomal proteins were also determined. Immunofluorescence assays showed that autophagy was increased following infection with all three types of pathogens, while only L. m infection stimulated lysosomal biosynthesis. Combined with more detailed immunoblotting and immunofluorescence analyses, these results suggest that macrophage lysosomes function as a signaling center in regulating immune responses to microbial infection.
HostingRepository	PRIDE
AnnounceDate	2017-04-24
AnnouncementXML	Submission_2017-04-24_00:22:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-15 02:16:29	ID requested
⏵ 1	2017-04-24 00:23:00	announced

Gao Y, Chen Y, Zhan S, Zhang W, Xiong F, Ge W, Comprehensive proteome analysis of lysosomes reveals the diverse function of macrophages in immune responses. Oncotarget, 8(5):7420-7440(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: proteomics, macrophage lysosome, innate immunity, pathogen

wei.ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Dongdan Santiao 5#, Dongcheng district, Beijing 100005, China
contact email	wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002915
     1. Label: PRIDE project
     2. Name: Comprehensive proteome analysis reveals the central role of macrophage lysosomes in regulating immune responses