PXD002911

PXD002911 is an original dataset announced via ProteomeXchange.

Title	The pathogenesis of Guillain-Barre syndrome: immunological profiling of physiological fluids
Description	Acute inflammatory demyelinating polyneuropathy (AIDP) - one of the forms of Guillain-Barre syndrome (GBS) - is a rare and severe disorder of the peripheral nervous system that also has an unknown etiology. One of the hallmarks of AIDP pathogenesis is a significantly elevated cerebrospinal fluid (CSF) protein level in comparison with the peptidome/proteome profile of a representative number of CSF samples obtained from AIDP and multiple sclerosis (MS) patients and control patients. In total, 2355 peptide fragments related to 795 proteins were found in CSF samples from AIDP patients, 1066 peptide fragments of 253 proteins in samples from MS patients, and 774 peptide fragments of 220 proteins from control patients. Proteomic studies detected 1103 proteins in the control cohort and 1402 proteins in the AIDP cohort. Our dataset suggests that the proteome of the CSF from AIDP and control patients overlaps by as much as 67%, whereas the peptidome is only 21.5% identical. Comparison of the peptidomic and proteomic data revealed that 80% of the peptides in the CSF of AIDP patients correspond to proteins that are not present in proteomic dataset. Importantly, a considerable number of differentially represented peptides are related to the proteins involved in the arrangement of the axonal domains. The observed peptidomic dataset indicates that specific proteins, which participate in the arrangement of the myelin sheath in the nodes of Ranvier, are degraded in the course of AIDP. Here, we show that the proteins that are overrepresented in the CSF of AIDP patients are partially linked with defensive responses to bacteria and viruses. These observations are in line with the upregulation of CSF cytokines associated with innate immunity, which in turn surprisingly suggests that the destruction of axons is not accompanied by any indications of a distinct adaptive autoimmune process. We believe that the presented proteomic and peptidomic dataset of the CSF from AIDP patients will provide useful information regarding the mechanisms of AIDP pathogenesis in comparison with MS, which is the prolonged but incurable autoimmune degradation of the central nervous system.
HostingRepository	PRIDE
AnnounceDate	2016-05-18
AnnouncementXML	Submission_2016-05-18_05:08:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002911
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgij Arapidi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Carboxymethyl; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-15 01:36:12	ID requested
⏵ 1	2016-05-18 05:09:00	announced

Ziganshin RH, Ivanova OM, Lomakin YA, Belogurov AA, Kovalchuk SI, Azarkin IV, Arapidi GP, Anikanov NA, Shender VO, Piradov MA, Suponeva NA, Vorobyeva AA, Gabibov AG, Ivanov VT, Govorun VM, The Pathogenesis of the Demyelinating Form of Guillain-Barre Syndrome (GBS): Proteo-peptidomic and Immunological Profiling of Physiological Fluids. Mol Cell Proteomics, 15(7):2366-78(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Guillain-Barre syndrome, AIDP, cerebrospinal fluid, immunoglobulins

Georgij Arapidi
contact affiliation	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
contact email	arapidi@gmail.com
lab head
Georgij Arapidi
contact affiliation	IBCH RAS
contact email	arapidi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD002911

PRIDE project URI

• PRIDE
  1. PXD002911
     1. Label: PRIDE project
     2. Name: The pathogenesis of Guillain-Barre syndrome: immunological profiling of physiological fluids