PXD002905

PXD002905 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mouse_Hippocampus_LCMSMS_IEF_Pooling
Description	Protein extract from a single mouse hippocampus was enzymatically digested and fractionated by isoelectric focusing. Aliquots of fractions were pooled to make fewer, more complex samples. The unfractionated lysate, fractions, and pooled fractions were subjected to liquid chromatography–mass spectrometry analysis. Samples consisting of many individual fractions had more protein identifications and quantified proteins with more spectral counts and greater precision than protein extract that was unfractionated or pooled into fewer LC-MS/MS samples.
HostingRepository	PRIDE
AnnounceDate	2016-05-09
AnnouncementXML	Submission_2016-05-09_01:53:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Bundy
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-14 06:24:05	ID requested
⏵ 1	2016-05-09 01:53:26	announced

Publication List

Bundy JL, Inouye BD, Mercer RS, Nowakowski RS, Fractionation-dependent improvements in proteome resolution in the mouse hippocampus by IEF LC-MS/MS. Electrophoresis, 37(14):2054-62(2016) [pubmed]

Bundy JL, Inouye BD, Mercer RS, Nowakowski RS, Fractionation-dependent improvements in proteome resolution in the mouse hippocampus by IEF LC-MS/MS. Electrophoresis, 37(14):2054-62(2016) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: LC-MSMS, brain, hippocampus, mouse, isoelectric focusing

curator keyword: Biological

submitter keyword: LC-MSMS, brain, hippocampus, mouse, isoelectric focusing

Contact List

Richard Nowakowski
contact affiliation	Florida State University College of Medicine, Biomedical Sciences
contact email	richard.nowakowski@med.fsu.edu
lab head
Joseph Bundy
contact affiliation	Florida State University College of Medicine
contact email	josephlbundy@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD002905
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD002905

PRIDE project URI

Repository Record List

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