PXD002901

PXD002901 is an original dataset announced via ProteomeXchange.

Title	Protein turnover during C. elegans aging
Description	Protein turnover rates severely decline in aging organisms, including C. elegans. However, limited information is available on turnover dynamics at the individual protein level during aging. We followed changes in protein turnover at one-day resolution using a multiple-pulse 15N-labeling and accurate mass spectrometry approach. Forty percent of the proteome shows gradual slowdown in turnover with age, while only few proteins show increased turnover. Decrease in protein turnover was consistent for the minority of functional protein subsets, including tubulins and vitellogenins, while for most functionally related protein pools randomly diverging turnover patterns were observed with age. Our data suggests severe dysregulation of protein turnover of the translation machinery, whereas protein turnover of UPS and antioxidant systems are well-preserved over time. Hence, we presume that maintenance of quality control mechanisms is a protective strategy in aging worms, although the ultimate proteome collapse is inescapable.
HostingRepository	PRIDE
AnnounceDate	2017-07-11
AnnouncementXML	Submission_2017-07-11_05:58:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002901
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vlad Petyuk
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-14 01:49:31	ID requested
⏵ 1	2017-07-11 05:58:22	announced

Dhondt I, Petyuk VA, Bauer S, Brewer HM, Smith RD, Depuydt G, Braeckman BP, . Mol Cell Proteomics, 16(9):1621-1633(2017) [pubmed]

curator keyword: Biological

submitter keyword: c. elegans, aging, protein turnover

Vlad Petyuk
contact affiliation	Pacific Northwest National Lab
contact email	vladislav.petyuk@pnnl.gov
lab head
Vlad Petyuk
contact affiliation	Pacific Northwest National Lab
contact email	vladislav.petyuk@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002901
     1. Label: PRIDE project
     2. Name: Protein turnover during C. elegans aging