PXD002899

PXD002899 is an original dataset announced via ProteomeXchange.

Title	CRM1 Exportome and nucleocytoplasmic partition
Description	CRM1 mediates one of the major nuclear export pathways with the broadest range of cargoes. So far, more than 100 structurally and functionally diverse CRM1 cargoes have been described. We employed affinity purification mass spectrometry for in depth characterization of CRM1 "exportome" in three model systems and we identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, ≈ 1000 from Xenopus oocytes and >1050 from human cells.I. Futher, we quantified the partitioning of ≈9600 proteoforms between nucleus and cytoplasm of Xenopus oocytes.
HostingRepository	PRIDE
AnnounceDate	2016-01-04
AnnouncementXML	Submission_2016-01-04_11:32:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samir Karaca
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-13 23:58:33	ID requested
⏵ 1	2016-01-04 11:32:06	announced

K, ı, rl, ı K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H, G, ö, rlich D, A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning. Elife, 4():(2015) [pubmed]

curator keyword: Biological

submitter keyword: Human, Xenopus, S.cerevisiae

Henning Urlaub and Dirk Görlich
contact affiliation	Henning Urlaub: Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Bioanalytics, Institute for Clinical Chemistry University Medical Center Göttingen, Göttingen, Dirk Goerlich:Department of Cellular Logistics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany;
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Samir Karaca
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	skaraca@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002899

PRIDE project URI

• PRIDE
  1. PXD002899
     1. Label: PRIDE project
     2. Name: CRM1 Exportome and nucleocytoplasmic partition