PXD002889

PXD002889 is an original dataset announced via ProteomeXchange.

Title	Stage-specific proteomes from Onchocerca ochengi, sister species of the human river blindness parasite, uncovers adaptations to a nodular lifestyle
Description	In spite of 40 years of control efforts, onchocerciasis (river blindness) remains one of the most important neglected tropical diseases, with 17 million people affected. The aetiological agent, Onchocerca volvulus, is a filarial nematode with a complex lifecycle involving several distinct stages in the definitive host and blackfly vector. The challenges of obtaining sufficient material have prevented high-throughput studies and the development of novel strategies for disease control and diagnosis. Here, we utilise the closest relative of O. volvulus, the bovine parasite Onchocerca ochengi, to compare stage-specific proteomes and host-parasite interactions within the secretome. We identified a total of 4,260 unique O. ochengi proteins from adult males and females, infective larvae, intrauterine microfilariae, and fluid from intradermal nodules. In addition, 135 proteins were detected from the obligate Wolbachia symbiont. Observed protein families that were enriched in all whole body extracts relative to the complete search database included immunoglobulin-domain proteins, whereas redox and detoxification enzymes and proteins involved in intracellular transport displayed stage-specific overrepresentation. Unexpectedly, the larval stages exhibited enrichment for several mitochondrial-related protein families, including members of peptidase family M16 and proteins which mediate mitochondrial fission and fusion. Quantification of proteins across the lifecycle using the Hi 3 approach supported these qualitative analyses. In nodule fluid, we identified 94 O. ochengi secreted proteins, including two homologs of transforming growth factor  and a second member of a novel 6 ShK toxin domain family, which was originally identified from a model filarial nematode (Litomosoides sigmodontis). Strikingly, the 498 bovine proteins identified in nodule fluid were strongly dominated by antimicrobial proteins, especially cathelicidins. This first high-throughput analysis of an Onchocerca spp. proteome highlights its profound complexity and emphasises the extremely close relationship between O. ochengi and O. volvulus. The insights provided here provide new candidates for vaccine development, drug targeting and diagnostic biomarkers.
HostingRepository	PRIDE
AnnounceDate	2016-05-31
AnnouncementXML	Submission_2016-05-31_04:52:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002889
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Onchocerca ochengi (Filarial nematode worm); NCBI TaxID: 42157;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-10 02:56:16	ID requested
⏵ 1	2016-05-31 04:52:01	announced

Armstrong SD, Xia D, Bah GS, Krishna R, Ngangyung HF, LaCourse EJ, McSorley HJ, Kengne-Ouafo JA, Chounna-Ndongmo PW, Wanji S, Enyong PA, Taylor DW, Blaxter ML, Wastling JM, Tanya VN, Makepeace BL, Stage-specific Proteomes from Onchocerca ochengi, Sister Species of the Human River Blindness Parasite, Uncover Adaptations to a Nodular Lifestyle. Mol Cell Proteomics, 15(8):2554-75(2016) [pubmed]

curator keyword: Biological

submitter keyword: Onchocerca ochengi, LC-MSMS, River Blindness

Julian Hiscox
contact affiliation	Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool. Liverpool, UK.
contact email	julian.hiscox@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD002889

PRIDE project URI

• PRIDE
  1. PXD002889
     1. Label: PRIDE project
     2. Name: Stage-specific proteomes from Onchocerca ochengi, sister species of the human river blindness parasite, uncovers adaptations to a nodular lifestyle