PXD002888

PXD002888 is an original dataset announced via ProteomeXchange.

Title	Construction and analysis of the testis/sperm-enriched interaction network: identification of potential PPP1CC2 complexes as targets for male contraception
Description	Phosphoprotein phosphatase 1 catalytic subunit gamma 2 (PPP1CC2), a PPP1 isoform restricted to testicular germ cells and spermatozoa, is essential for completion of spermatogenesis and highly similar PPP1C isoforms are capable of compensating for the loss of Ppp1cc in every tissue except in testis. In addition, inhibition of the enzymatic activity of PPP1C in spermatozoa stimulates motility. The key to understand the regulation of PPP1CC2 lies in the identification and characterization of its interacting partners. By integrating tissue-specific protein expression and protein-protein interaction data, we identified testis/sperm-enriched/specific PPP1CC complexes and potential key PPP1 interacting proteins (PIPs) for male reproduction. We constructed a testis/sperm-enriched network, analyzed several topological properties and specified the biological/physiological properties of the proteins in the network. Proteins were classified into modules according to their topological features and functional annotations, which clarified the mechanisms underlying testis and sperm function and, more specifically, the role of PPP1CC in spermatogenesis and sperm motility. Previously uncharacterized PIPs, such as AKAP4, were identified based on analyses of the interaction network. The PPP1CC2 interaction with AKAP4 in human sperm was also validated by different methods and the complex was proposed as potential targets for male contraception due to its significance in spermatozoa motility.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:50.433.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002888
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pieter-Jan De Bock
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Gln->pyro-Glu
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-10 02:30:01	ID requested
1	2022-02-28 02:52:57	announced
⏵ 2	2023-11-14 08:48:51	announced	2023-11-14: Updated project metadata.

10.6019/PXD002888;

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Biological

submitter keyword: Human, sperm, PPP1CC2

Kris Gevaert
contact affiliation	Ghent University VIB Belgium
contact email	kris.gevaert@vib-ugent.be
lab head
Pieter-Jan De Bock
contact affiliation	Biochemistry
contact email	pieter-jan.debock@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002888
     1. Label: PRIDE project
     2. Name: Construction and analysis of the testis/sperm-enriched interaction network: identification of potential PPP1CC2 complexes as targets for male contraception