PXD002882 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Altered Intestinal Microbiota-Host Mitochondria Crosstalk in New Onset Crohn’s Disease |
Description | Intestinal microbial dysbiosis is associated with Crohn’s disease (CD). However, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. To evaluate causality and mechanisms of disease, we conducted a systems level study of the interactions between the gut microbiota and host in new-onset pediatric patients. We report an altered host proteome in CD patients indicative of impaired mitochondrial functions. A downregulation of mitochondrial proteins implicated in H2S detoxification was observed, while the relative abundance of H2S microbial producers was increased. Network correlation analysis identified Atopobium parvulum as the central hub of H2S producers. Gnotobiotic and conventionalized colitis-susceptible interleukin-10-deficient (Il10-/-) mice demonstrated that A. parvulum induced colitis, a phenotype requiring the presence of the intestinal microbiota. Administration of bismuth, a H2S scavenger, prevented A. parvulum-induced colitis in Il10-/- mice. This study identified host-microbiota interactions that are disturbed in CD patients providing mechanistic insights on CD pathogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:25:30.087.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amanda Starr |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-09 05:24:14 | ID requested | |
1 | 2016-09-29 13:13:33 | announced | |
2 | 2019-02-12 07:54:17 | announced | Updated publication reference for PubMed record(s): 27876802. |
⏵ 3 | 2024-10-22 04:25:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/ncomms13419; |
Mottawea W, Chiang CK, M, ü, hlbauer M, Starr AE, Butcher J, Abujamel T, Deeke SA, Brandel A, Zhou H, Shokralla S, Hajibabaei M, Singleton R, Benchimol EI, Jobin C, Mack DR, Figeys D, Stintzi A, Altered intestinal microbiota-host mitochondria crosstalk in new onset Crohn's disease. Nat Commun, 7():13419(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Crohn's Disease, Microbiota, Gut,IBD |
Contact List
Alain Stintzi |
contact affiliation | Department of Biochemistry, Microbiology and Immunology University of Ottawa, 451 Smyth Rd. Ottawa, Ontario, Canada, K1H 8M5 |
contact email | astintzi@uottawa.ca |
lab head | |
Amanda Starr |
contact affiliation | University of Ottawa |
contact email | amanda.e.starr@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002882
- Label: PRIDE project
- Name: Altered Intestinal Microbiota-Host Mitochondria Crosstalk in New Onset Crohn’s Disease