PXD002882

PXD002882 is an original dataset announced via ProteomeXchange.

Title	Altered Intestinal Microbiota-Host Mitochondria Crosstalk in New Onset Crohn’s Disease
Description	Intestinal microbial dysbiosis is associated with Crohn’s disease (CD). However, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. To evaluate causality and mechanisms of disease, we conducted a systems level study of the interactions between the gut microbiota and host in new-onset pediatric patients. We report an altered host proteome in CD patients indicative of impaired mitochondrial functions. A downregulation of mitochondrial proteins implicated in H2S detoxification was observed, while the relative abundance of H2S microbial producers was increased. Network correlation analysis identified Atopobium parvulum as the central hub of H2S producers. Gnotobiotic and conventionalized colitis-susceptible interleukin-10-deficient (Il10-/-) mice demonstrated that A. parvulum induced colitis, a phenotype requiring the presence of the intestinal microbiota. Administration of bismuth, a H2S scavenger, prevented A. parvulum-induced colitis in Il10-/- mice. This study identified host-microbiota interactions that are disturbed in CD patients providing mechanistic insights on CD pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:25:30.087.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amanda Starr
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-09 05:24:14	ID requested
1	2016-09-29 13:13:33	announced
2	2019-02-12 07:54:17	announced	Updated publication reference for PubMed record(s): 27876802.
⏵ 3	2024-10-22 04:25:30	announced	2024-10-22: Updated project metadata.

10.1038/ncomms13419;

Mottawea W, Chiang CK, M, ü, hlbauer M, Starr AE, Butcher J, Abujamel T, Deeke SA, Brandel A, Zhou H, Shokralla S, Hajibabaei M, Singleton R, Benchimol EI, Jobin C, Mack DR, Figeys D, Stintzi A, Altered intestinal microbiota-host mitochondria crosstalk in new onset Crohn's disease. Nat Commun, 7():13419(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Crohn's Disease, Microbiota, Gut,IBD

Alain Stintzi
contact affiliation	Department of Biochemistry, Microbiology and Immunology University of Ottawa, 451 Smyth Rd. Ottawa, Ontario, Canada, K1H 8M5
contact email	astintzi@uottawa.ca
lab head
Amanda Starr
contact affiliation	University of Ottawa
contact email	amanda.e.starr@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD002882

PRIDE project URI

• PRIDE
  1. PXD002882
     1. Label: PRIDE project
     2. Name: Altered Intestinal Microbiota-Host Mitochondria Crosstalk in New Onset Crohn’s Disease