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PXD002882

PXD002882 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAltered Intestinal Microbiota-Host Mitochondria Crosstalk in New Onset Crohn’s Disease
DescriptionIntestinal microbial dysbiosis is associated with Crohn’s disease (CD). However, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. To evaluate causality and mechanisms of disease, we conducted a systems level study of the interactions between the gut microbiota and host in new-onset pediatric patients. We report an altered host proteome in CD patients indicative of impaired mitochondrial functions. A downregulation of mitochondrial proteins implicated in H2S detoxification was observed, while the relative abundance of H2S microbial producers was increased. Network correlation analysis identified Atopobium parvulum as the central hub of H2S producers. Gnotobiotic and conventionalized colitis-susceptible interleukin-10-deficient (Il10-/-) mice demonstrated that A. parvulum induced colitis, a phenotype requiring the presence of the intestinal microbiota. Administration of bismuth, a H2S scavenger, prevented A. parvulum-induced colitis in Il10-/- mice. This study identified host-microbiota interactions that are disturbed in CD patients providing mechanistic insights on CD pathogenesis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:25:30.087.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAmanda Starr
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-09 05:24:14ID requested
12016-09-29 13:13:33announced
22019-02-12 07:54:17announcedUpdated publication reference for PubMed record(s): 27876802.
32024-10-22 04:25:30announced2024-10-22: Updated project metadata.
Publication List
10.1038/ncomms13419;
Mottawea W, Chiang CK, M, ü, hlbauer M, Starr AE, Butcher J, Abujamel T, Deeke SA, Brandel A, Zhou H, Shokralla S, Hajibabaei M, Singleton R, Benchimol EI, Jobin C, Mack DR, Figeys D, Stintzi A, Altered intestinal microbiota-host mitochondria crosstalk in new onset Crohn's disease. Nat Commun, 7():13419(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Crohn's Disease, Microbiota, Gut,IBD
Contact List
Alain Stintzi
contact affiliationDepartment of Biochemistry, Microbiology and Immunology University of Ottawa, 451 Smyth Rd. Ottawa, Ontario, Canada, K1H 8M5
contact emailastintzi@uottawa.ca
lab head
Amanda Starr
contact affiliationUniversity of Ottawa
contact emailamanda.e.starr@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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