PXD002880

PXD002880 is an original dataset announced via ProteomeXchange.

Title	mRNA binding
Description	RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.
HostingRepository	PRIDE
AnnounceDate	2015-09-11
AnnouncementXML	Submission_2015-09-11_07:26:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophia Foehr
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-09 01:14:19	ID requested
⏵ 1	2015-09-11 07:26:31	announced

Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW, Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell, 149(6):1393-406(2012) [pubmed]

curator keyword: Biological

submitter keyword: mRNA binding

Jeroen Krijgsveld
contact affiliation	EMBL
contact email	jeroen.krijgsveld@embl.de
lab head
Sophia Foehr
contact affiliation	EMBL
contact email	foehr@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD002880

PRIDE project URI

• PRIDE
  1. PXD002880
     1. Label: PRIDE project
     2. Name: mRNA binding