PXD002875

PXD002875 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide quantitative multiplexed profiling of protein expression: Carbon source dependency in S. cerevisiae
Description	The global proteomic alterations in the budding yeast Saccharomyces cerevisiae due to differences in carbon sources can be comprehensively examined using mass spectrometry-based multiplexing strategies. Here we investigate changes in the S. cerevisiae proteome resulting from cultures grown in minimal media using galactose, glucose, or raffinose as the carbon source. We used a TMT 9-plex strategy to determine alterations in relative protein abundance due to a particular carbon source, in triplicate, thereby permitting subsequent statistical analyses. We quantified over 4700 proteins across all 9 samples, of which 1003 demonstrated statistically significant differences in abundance in at least one condition. The majority of altered proteins were classified as functioning in metabolic processes and as having cellular origins of plasma membrane and mitochondria. In contrast, proteins remaining relatively unchanged in abundance included those having nucleic acid-related processes, such as transcription and RNA processing. In addition, the comprehensiveness of the dataset enabled the analysis of subsets of functionally-related proteins, such as phosphatases, kinases, and transcription factors. Moreover, alterations in protein abundance levels of full sets of proteins that comprise distinct biological pathways, such as galactose metabolism and the TCA cycle, can be examined collectively. As a resource, these data can be mined further in efforts to understand better the roles of carbon source fermentation in yeast metabolic pathways and potentially utilize observed alterations for industrial applications, such as biofuel feedstock production.
HostingRepository	PRIDE
AnnounceDate	2016-04-20
AnnouncementXML	Submission_2016-04-20_03:42:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-08 06:07:17	ID requested
⏵ 1	2016-04-20 03:42:21	announced

Paulo JA, O'Connell JD, Gaun A, Gygi SP, Proteome-wide quantitative multiplexed profiling of protein expression: carbon-source dependency in Saccharomyces cerevisiae. Mol Biol Cell, 26(22):4063-74(2015) [pubmed]

curator keyword: Biological

submitter keyword: : yeast, glucose, galactose, raffinose, TMT, Fusion

Joao A. Paulo
contact affiliation	Harvard Medical School, Boston, MA, USA
contact email	joao_paulo@hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002875
     1. Label: PRIDE project
     2. Name: Proteome-wide quantitative multiplexed profiling of protein expression: Carbon source dependency in S. cerevisiae