PXD002872

PXD002872 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Shotgun analysis of nuclear proteins from mouse brain
Description	Nuclei were isolated from the mouse brain. Proteins were extracted with addition of Bensonase and cleaned up by acetone precipitation. Proteis were resuspended in 50 mM ammonium bicarbonate and PPS Silent Surfactant. Samples were sonicated on ice to improve the solubility of proteins, then alkylated with IAA and digested with trypsin. Peptides were analyzed by LC-MS/MS using the UltiMate 3000RS nano-System coupled with microTOF_QII.
HostingRepository	MassIVE
AnnounceDate	2016-06-29
AnnouncementXML	Submission_2016-06-29_06:50:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jankowska
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl; Oxidation; Deamidated
Instrument	micrOTOF-Q II

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-08 04:07:37	ID requested
⏵ 1	2016-06-29 06:50:09	announced

Publication List

Jankowska U, Latosinska A, Skupien-Rabian B, Swiderska B, Dziedzicka-Wasylewska M, Kedracka-Krok S, Optimized procedure of extraction, purification and proteomic analysis of nuclear proteins from mouse brain. J Neurosci Methods, 261():1-9(2016) [pubmed]

Jankowska U, Latosinska A, Skupien-Rabian B, Swiderska B, Dziedzicka-Wasylewska M, Kedracka-Krok S, Optimized procedure of extraction, purification and proteomic analysis of nuclear proteins from mouse brain. J Neurosci Methods, 261():1-9(2016) [pubmed]

Keyword List

submitter keyword: nuclear proteins, mouse brain, nucleus

submitter keyword: nuclear proteins, mouse brain, nucleus

Contact List

Sylwia Kedracka-Krok
lab head
Jankowska
contact affiliation	Jagiellonian University
contact email	urszula.jankowska@uj.edu.pl
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079301

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079301